"Cytiva"
Disposable columns, prepacked, Sepharose™ GST bulk kit
Supplier: Cytiva
GST bulk kit is designed for small-scale manual purification of GST-tagged proteins using either column chromatography or batch method. Facilitates optimization studies of expression, solubility conditions and purification parameters of GST-tagged proteins.
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Gel filtration columns, HiPrep™ with Sephacryl® S-100 HR
Supplier: Cytiva
HiPrep Sephacryl S-100 HR are prepacked gel filtration columns for preparative separation of biomolecules from up to 13 ml sample volume.
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Affinity chromatography media, Lentil Lectin Sepharose™ 4B
Supplier: Cytiva
Lentil lectin is a metalloprotein, containing Ca₂₊ and Mn₂₊, which binds reversibly to polysaccharides and glycoconjugates containing glucose or mannose type sugars.
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Phenyl Sepharose™ 6 Fast Flow (High Sub) Hydrophobic Interaction Chromatography Media
Supplier: Cytiva
Phenyl Sepharose 6 Fast Flow is a well established, standard aromatic hydrophobic interaction chromatography (HIC) resin for capture and intermediate purification requiring low to resin hydrophobicity
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Ion exchange chromatography media, Q Sepharose™ High Performance
Supplier: Cytiva
Q Sepharose™ High Performance is composed of crosslinked agarose beads with a mean diameter of 34 µm, modified with quaternary (Q) strong anion exchange groups.
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Affinity chromatography media, Ni Sepharose™ High Performance
Supplier: Cytiva
Ni Sepharose™ High Performance affinity media for high resolution, high capacity purification of histidine-tagged proteins by immobilised metal ion affinity chromatography (IMAC).
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Amersham CyDye DIGE fluors
Supplier: Cytiva
CyDye™ DIGE fluors are available as minimal and saturation labeling dyes. The minimal dyes are intended for general 2-D application use where sufficient amounts of sample are available. The saturation dyes from the Scarce Sample Labeling Kit are designed to be used for applications where only small amounts of sample are available, for example in Laser Capture Microdissection.
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Anti-IgG Goat Antibody (CyDye™ 700)
Supplier: Cytiva
Amersham CyDye NIR-labeled secondary antibodies enable quantitation of Western blots with a strong signal-to-noise ratio. Mulitplexed experiments allow for the detection of two proteins on a single blot. The use of total lane signals to normalize target protein data yield robust and reliable results.
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Marker per western blotting, Amersham ECL™ DualVue™
Supplier: Cytiva
I marker per western blotting Amersham ECL™ DualVue™ svolgono due funzioni fondamentali. Anzitutto, le proteine del marker pre-colorato confermano il trasferimento della proteina e definiscono chiaramente l'orientamento del blot. In secondo luogo, le proteine ricombinanti marcate vengono rilevate su pellicola o tramite imaging CCD insieme alle proteine di interesse, consentendo una determinazione del peso molecolare delle proteine target molto accurata. Questi marker vengono rilevati facilmente grazie ad uno specifico coniugato che elimina eventuali contaminazioni incrociate tra le proteine bersaglio e i marker.
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Monofunctional maleimide fluors, CyDye™
Supplier: Cytiva
Cy3 and Cy5 monofunctional maleimides are particularly suitable for the selective labelling of molecules containing free sulfhydryl groups, such as cysteine residues in proteins and peptides and oligonucleotides.
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DNA (Deoxyribonucleic acid) (from salmon testes), sonicated
Supplier: Cytiva
Genomic DNA sheared by sonication for use in hybridization experiments
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HiTrap™ Capto™ Octyl Hydrophobic Interaction Chromatography Column
Supplier: Cytiva
Capto™ Octyl are hydrophobic interaction chromatography (HIC) resins used in the capture and intermediate stages of protein purification.
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Antibody column package, Modern Protein A (2-step purification protocol)
Supplier: Cytiva
The Antibody column package includes modern protein A affinity and size exclusion chromatography columns to obtain highly pure human IgG.
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Affinity chromatography columns, HiTrap™ KappaSelect
Supplier: Cytiva
KappaSelect is an affinity medium designed for the purification of human Fab (kappa) fragments.
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illustra™ bacteria genomicPrep Mini Spin Kit
Supplier: Cytiva
The illustra™ bacteria genomicPrep Mini Spin Kit is designed for the rapid extraction and purification of high quality genomic DNA from gram-positive and gram-negative bacteria. The kit can be used to isolate genomic DNA from a wide range of bacterial strains such as DH5a, TOP10, JM109, Bacillus subtilis, etc. The illustra™ bacteria genomicPrep Mini Spin Kit produces highly pure and intact genomic DNA with A260/A280 values of 1,75 to 1,85.
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Isoelectric focusing system, Ettan™ IPGphor™ 3
Supplier: Cytiva
Ettan™ IPGphor™ 3 is a fully integrated isoelectric focusing (IEF) system optimised to deliver high throughput, speed, reproducibility, and high protein-loading capacity. Ettan™ IPGphor™ 3 is optimised for easy handling of CyDye™ labelled proteins and other light-sensitive samples, and provides temperature control critical for reproducibility in 2-D DIGE experiments.
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