"Cytiva"

Supplier: Cytiva

The 2-D Clean-Up Kit is designed to prepare samples for 2-D electrophoresis that otherwise produce poor 2-D spot-maps due to high conductivity, high levels of interfering substances, or low protein concentration. The reagents quantitatively precipitate proteins while leaving interfering substances, such as detergents, salts, lipids, phenolics, and nucleic acids, in solution.

Supplier: Cytiva

PD-10 Desalting Columns contain Sephadex G-25 resin for rapid buffer exchange, desalting, and removal of small contaminants (salts, dyes, radioactive labels) from samples using gravity flow or centrifugation.

Supplier: Cytiva

HisTrap FF Crude columns are high binding capacity IMAC columns for his-tagged protein purification when scalability is needed.

Supplier: Cytiva

HiTrap Q FF is prepacked with Q Sepharose Fast Flow, and is a strong anion exchanger for small-scale protein purifications as well as screening of binding and elutions conditions.

Supplier: Cytiva

HiTrap Heparin HP columns are designed for high-resolution purification of DNA-binding proteins, coagulation factors, and other plasma proteins.

Supplier: Cytiva

Polyetheretherketone tubing (PEEK) is intended for use with AKTA systems.

Supplier: Cytiva

Urea

Supplier: Cytiva

DEAE Sepharose™ Fast Flow is part of the Sepharose™ Fast Flow ion exchange platform. It is composed of cross-linked 6% agarose beads, with diethylaminoethyl (DEAE) weak anion exchange groups. Scale-up with DEAE Sepharose™ Fast Flow is straight forward.

Supplier: Cytiva

Superdex 200 Increase size exclusion chromatography columns are well suited for high-resolution analysis and small-scale purification of monoclonal antibodies (mAb) and other biomolecules with Mr~ 10000 to 600000.

Supplier: Cytiva

Amersham™ Protran® membranes are manufactured from 100% pure nitrocellulose to ensure the highest binding capacity possible. They are compatible with a variety of detection methods, including isotopic, chemiluminescent, colorimetric and fluorescent. No methanol pre-wetting step is required, making this the membrane of choice for proteins which prefer aqueous environments. Prior to transfer the membrane is simply wetted with water, and then placed in the transfer buffer. No other pretreatment steps are necessary.

Supplier: Cytiva

NHS-activated Sepharose™ 4 Fast Flow is composed of cross-linked 4% agarose beads, NHS activated for coupling amino-containing proteins and peptides in process-scale applications. NHS (N-hydroxysuccinimide) coupling forms a chemically stable amide bond with ligands containing primary amino groups. The resin provides a spacer arm to the coupled ligand and suitable for immobilising smaller protein and peptide ligands.

Supplier: Cytiva

HiTrap™ Protein A HP is a pre-packed, ready to use Protein A Sepharose™ High Performance column for preparative purification of monoclonal and polyclonal antibodies from ascites, serum and cell culture supernatants.

Supplier: Cytiva

PD SpinTrap G-25 is a single-use column for cleanup of large biomolecules.

Supplier: Cytiva

CNBr-activated Sepharose™ 4B is pre-activated media used for coupling antibodies or other large proteins containing -NH₂ groups to the Sepharose™ media, by the cyanogen bromide method, without an intermediate spacer arm.

Supplier: Cytiva

illustra™ AutoSeq™ G-50 columns are specifically designed to remove fluorescent dye-terminators from sequencing reactions prior to analysis on automated sequencers. Effective purification is essential for high quality sequencing results since residual dye-terminators can obscure data. illustra™ AutoSeq™ G-50 consists of MicroSpin™ columns containing Sephadex™ G-50, pre-equilibrated in double-distilled water. This is important because even the small amount of salt in traditional buffers (such as TE buffer) can cause electrophoretic artifacts on salt-sensitive automated sequencers.

Supplier: Cytiva

Ethylenetetrafluorethylene tubing (ETFE) is designed for use with AKTA systems.