"Biotium"
Fluorometric polymerase activity assay kit, EvaEZ™
Supplier: Biotium
EvaEZ™ Fluorometric Polymerase Activity Assay Kit provides an easy and accurate way to determine the activity of a nucleic acid polymerase without using radioisotopes.
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PMA (Propidium monoazide) DNA/RNA binding dye
Supplier: Biotium
PMA (propidium monoazide) is a high-affinity photoreactive DNA binding dye invented by scientists at Biotium for viability PCR of bacteria and other organisms. The dye is weakly fluorescent by itself but becomes more fluorescent after binding to nucleic acids.
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Propidium iodide 1 mg/ml in water
Supplier: Biotium
Propidium iodide (PI) is a membrane-impermeant nucleic acid intercalator. The dye is commonly used to selectively stain dead cells in a cell population and also used as a counterstain in multicolor fluorescent imaging.
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PMAxx™ dye 20 mM in water
Supplier: Biotium
PMAxx™ dye is a DNA modifier used for viability PCR (v-PCR). V-PCR is a powerful PCR-based, culture-independent, method for rapid and sensitive quantification of microbial viability.
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SYBR® Green I 10000x in DMSO nucleic acid gel stain
Supplier: Biotium
Thiazole green, which is structurally identical to SYBR® green I nucleic acid gel stain, is one of the most sensitive stains available for detecting double-stranded DNA (dsDNA) in agarose, polyacrylamide gels and qPCR.
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Decontamination bags, activated charcoal
Supplier: Biotium
Activated charcoal retains ethidium bromide, GelRed™ and GelGreen™ efficiently in a convenient bag for decontamination of used gel staining solutions. Each bag has a total capacity of at least 10 mg ethidium bromide.
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Wheat germ agglutinin (WGA) conjugates
Supplier: Biotium
A wheat germ agglutinin (WGA) lectin labelled with Biotium’s CF® Dyes or HRP. It is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine moieties of glycoproteins. As a result, WGA conjugates will label yeast bud scars and the cell membrane of gram-positive bacteria and mammalian cells.
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Fluorescence antifade mounting media and CoverGrip™ coverslip sealant, EverBrite™
Supplier: Biotium
EverBrite™ mounting media are anti-fade mountants for preserving fluorescence during microscopy. EverBrite™ mounting media prevent rapid photobleaching of fluorescein, rhodamine, and cyanine based dyes and are optimally formulated for use with Biotum’s CF™ dye conjugates. Unlike Vectashield® EverBrite™ mounting media is compatible with Cy® dyes such as Cy®2, Cy®3 and Cy®5. EverBrite™ mounting medium is a wet-set medium while EverBrite™ hardset mounting medium cures overnight to form a hard permanent seal with the coverslip, eliminating the need to seal coverslip edges with nail polish or other sealants. Both formulations are available with DAPI for blue fluorescent nuclear counterstaining without an additional staining step.
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Anti-KRT19 Mouse Monoclonal Antibody (CF488A) [clone: KRT19/799]
Supplier: Biotium
Recognizes a protein of 40 kDa, identified as cytokeratin-19 (CK19), which is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50%-60% of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer.
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One-Step Blue® protein gel stain
Supplier: Biotium
One-Step Blue® is a ready-to-use protein gel staining solution. It produces fast (5-60 min) protein staining in a single step without fixation or washing.
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Hydroxystilbamidine bis(methanesulphonate) 4% in water fluorescent dye, per istologia
Supplier: Biotium
Hydroxystilbamidine (also called Fluoro-Gold™) has been used extensively as a retrograde tracer for neurons and also a histochemical stain. The 4% solution in deionized H2O is a convenient, ready-to-use form..
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Nucleic acid stains, PAGE GelRed™
Supplier: Biotium
Stains specifically designed for DNA in polyacrylamide gels.
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Coloranti gel per acidi nucleici, GelRed™ e GelGreen™
Supplier: Biotium
GelRed e GelGreen sono coloranti fluorescenti per acidi nucleici che coniugano elevata sensibilità, bassa tossicità ed eccezionale stabilità.
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EvaGreen® Plus dye, 20X in Water
Supplier: Biotium
EvaGreen® Plus Dye has an improved signal-to-noise compared to original EvaGreen® dye, for higher sensitivity in DNA amplification applications.
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PMA-Lite™ 2.0 LED Photolysis Device
Supplier: Biotium
The PMA-Lite™ 2.0 LED photolysis device is a light-weight LED light box specifically designed for optimal photoactivation of samples treated with propidium monoazide (PMA), PMAxx™ or ethidium monoazide (EMA) in viability PCR applications.
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Dimetilsolfossido, anidro ≥99.9%
Supplier: Biotium
Anhydrous DMSO (dimethyl sulfoxide; methyl sulfoxide) is recommended for preparing stock solutions.



