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Tetrabutylammonium chloride 95% tech. contains also bromide

Tetrabutylammonium chloride 95% tech. contains also bromide

Supplier: Thermo Fisher Scientific

Tetrabutylammonium chloride 95% tech. contains also bromide

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Hydroxystilbamidine bis(methanesulphonate) fluorescent dye, per istologia

Hydroxystilbamidine bis(methanesulphonate) fluorescent dye, per istologia

Supplier: Biotium

Hydroxystilbamidine (also called Fluoro-Gold™) has been used extensively as a retrograde tracer for neurons and also a histochemical stain. Fluoro-Gold™ can undergo retrograde axonal transport, it shows extensive filling of dendrites, and has a high resistance to fading.

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Anti-ATP1B1 Mouse Monoclonal Antibody [clone: 464.8 (also known as 8A)]

Supplier: OriGene

Anti-ATP1B1 Mouse Monoclonal Antibody [clone: 464.8 (also known as 8A)]

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Furaptra (Mag-Fura-2) tetrasodium salt ≥95% (by HPLC) fluorescent indicator, for calcium determination

Supplier: Biotium

Mag-Fura-2 is a UV-excitable fluorescent indicator for magnesium with a Kd of 1.9 mM. Similar to Fura-2, the excitation wavelength of Mag-Fura-2 undergoes a blue shift from 369 nm to 330 nm. Mag-Fura-2 also responds to Ca2+ but with a significantly higher Kd than Fura-2 for Ca2+.

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Furaptra (Mag-Fura-2) tetrapotassium salt ≥95% (by HPLC) fluorescent indicator, for calcium determination

Supplier: Biotium

Mag-Fura-2 is a UV-excitable fluorescent indicator for magnesium with a Kd of 1.9 mM (1,2). Similar to Fura-2, the excitation wavelength of Mag-Fura-2 undergoes a blue shift from 369 nm to 330 nm. Mag-Fura-2 also responds to Ca2+ but with a significantly higher Kd than Fura-2 for Ca2+.

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Anti-ATP1B1 Mouse Monoclonal Antibody [clone: 464.8 (also known as 8A)]

Anti-ATP1B1 Mouse Monoclonal Antibody [clone: 464.8 (also known as 8A)]

Supplier: Abnova

Mouse monoclonal antibody raised against native ATP1B1.

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Coelenterazine Substrates for Renilla Luciferase and Apoaequorin

Supplier: Biotium

Coelenterazine and its analogs are luminescent enzyme substrates for apoaequorin and Renilla luciferase.

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Ethidium homodimer I used in nuclear staining

Supplier: Biotium

Ethidium Homodimer, first developed by Dr. Le Pecq and his colleagues, is a high-affinity fluorescent nucleic acid stain. It binds to both DNA and RNA in a sequence-independent manner and with a >30-fold fluorescence enhancement. The DNA binding of each Ethidium Homodimer covers four base pairs and is believed to occur by intercalation. Because the dye is highly positively charged, it cannot cross cell membranes to stain living cells. It is useful for detecting nucleic acids in solution, or for selectively staining dead cells with damaged plasma membranes.

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SynaptoGreen™ C18 (Equivalent to FM®3-25) membrane stain

SynaptoGreen™ C18 (Equivalent to FM®3-25) membrane stain

Supplier: Biotium

SynaptoGreen™ C18 (equivalent to FM®3-25) is a membrane stain with a chemical structure similar to other nerve terminal dyes, except that it has the longest lipophilic tail (C18). Because of its large size, it is used as a control for ion channel permeability of nerve terminal dyes.

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Bromobimane (mBBr) ≥97%, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Bromobimane is also known as monobromobimane. It is also a known probe for thiols and is a fluorescent reagent activated upon a photolysis reaction.

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Furaptra (Mag-Fura-2) AM Ester ≥95% (by TLC) fluorescent indicator, for calcium determination

Supplier: Biotium

Furaptra, AM ester is a membrane-permeant form of Furaptra (Mag-Fura-2). The indicator is loaded into cells via simple incubation

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Reactive oxygen species indicators dyes

Supplier: Biotium

Dihydrorhodamine 123 is the reduced form of rhodamine 123, which is a commonly used fluorescent mitochondrial dye. It is non-fluorescent, but it readily enters most of the cells and is oxidized by oxidative species or by cellular redox systems to the fluorescent rhodamine 123 that accumulates in mitochondrial membranes. It is useful for detecting reactive oxygen species including superoxide (in the presence of peroxidase or cytochrome c) and peroxynitrite. Dihydrorhodamine 123 dihydrochloride is functionally equivalent to dihydrorhodamine 123 but with increased stability toward air oxidation and light during storage. Dihydroethidium (also called hydroethidium) is the chemically reduced form of the commonly used DNA dye ethidium bromide. The probe is useful to detect oxidative activities in viable cells, including respiratory burst in phagocytes. Dihydroethidium itself has blue fluorescence (355/420 nm) in cells, while the oxidized form ethidium has red fluorescence (518/605 nm) upon DNA intercalation. H2DCFDA (2′,7′-Dichlorodihydrofluorescein diacetate) is a useful fluorogenic reagent to detect reactive oxygen intermediates in cells. On oxidation, H2DCFDA becomes the highly green fluorescent 2′,7′-dichlorofluorescein, 504/529 nm (end product).

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Sonde e coloranti fluorescenti per terminali nervosi

Sonde e coloranti fluorescenti per terminali nervosi

Supplier: Biotium

Nerve terminal probes are a series of fluorescent cationic styryl dyes developed to follow synaptic activity. These dyes have a lipophilic tail at one end and a hydrophilic cationically charged head group at the other end. The dyes vary in the number of carbons in the lipophilic tail (m) and the number of double bonds linking the two aromatic rings in the dye (n). AM dyes are similar to FM dyes, but contain an additional formaldehyde-fixable amino group in the cationic head.

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Organic reference standard, epsilon-Caprolactam, analytical standard, Supelco®

Supplier: Merck

ε-Caprolactam is used in the manufacture of polyamides. It is also used to produce nylon 6.

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Triethylammonium bicarbonate buffer, for HPLC, LiChropur™, Supelco®

Supplier: Merck

1M Buffer, also for use in ion-exchange chromatography and electrophoresis.

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5(6)-Carboxytetramethylrhodamine succinimidyl ester [5(6)-TAMRA SE] ≥98% (by TLC) amine-reactive fluorescent dye

Supplier: Biotium

5(6)-TAMRA SE (full name: 5-(and-6)-Carboxytetramethylrhodamine succinimidyl ester (mixed isomers)) is the amine reactive form of TAMRA, one of the most commonly used red fluorescent dyes. The succinimidyl ester reacts readily with primary or secondary amines under mild conditions.

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