The NEXTFLEX™ universal blockers block non-specific hybridisation, enhancing specificity and reducing sequencing costs. They are compatible with all ligation and tagmentation workflows for Illumina®.

• Effective in blocking single-index or dual-index libraries Compatible with all NEXTFLEX™ barcodes and all other ligation-based and tagmentation-based workflows for Illumina® sequencing platforms Simplifies and reduces the cost of target capture without sacrificing performance 96 barcode blockers available

During target enrichment, adapters can interact with the complementary adapter sequence strand of another library molecule. The NEXTFLEX™ universal blockers are designed to prevent this concatemerisation of library molecules. By blocking this complementary adapter sequence interaction, the number of on-target reads improves dramatically and the depth of enrichment increases. The NEXTFLEX™ universal blockers are designed for use with indexed adapters for Illumina sequencing platforms. They are effective in blocking single-indexed or dual-indexed libraries, without being affected by index length or the presence of a unique molecular index (UMI). The NEXTFLEX Universal Blockers are compatible with ligation-based workflows, such as NEXTFLEX™ or Illumina trusequence library prep kits. They are also compatible with tagmentation-based workflows, such as Illumina Nextera library prep kits.