Endothelial Cell Growth Medium, Advanced, is a sterile liquid culture medium designed for the cultivation of endothelial cells. Delivered in the form of a basal medium, it is well-suited for culturing Provitro human endothelial cells when supplemented with the provided mix components.

• Formulation is optimised for the initial seeding of 4000 cells/cm², allowing for growth up to confluence (approximately 90%)
• Use of feeder layers, matrix substrates, or other additives is not necessary

For freshly isolated cells, we recommend incorporating the antibiotic supplement to address potential reductions in proliferative activity. Endothelial cell proliferation medium is thoroughly tested after each production. All components are tested in a stringent biological assay. Each batch is checked for human endothelial cells proliferating characteristics. The cells cultured in endothelial cell growth medium are checked regarding their morphology, the adherence rate, the colony forming efficiency, and the population doubling time.

Storge: Store the supplemented endothelial cell proliferation medium in a dark environment at temperatures ranging from 4 to 8 °C for a maximum duration of one month. Avoid heating the medium beyond 37 °C and refrain from using uncontrolled heat sources, such as microwave appliances. When utilising a portion of the medium, extract the required amount from the bottle and warm it appropriately. Never freeze the medium, as this could result in elevated salt concentrations due to the separation of pure water during freezing, leading to irreversible damage.