Specifications
- Conf:96 Tests
- Durata del test:Multiple steps
- Tipo di test:Competitive
- Formato:Pre-coated
- Primary antibody reactivity:Rat
- Target protein:TSH beta
- Descrizione:Rat TSH beta ELISA kit
- Tiipo del campione:Serum, plasma, tissue homogenates and other biological fluids
- Reattività incrociata:Rat TSH beta ELISA Kit exhibits high specificity and excellent specificity for the detection of rat TSH beta. No significant cross-reactivity or interference between TSH beta and analogues was observed.
- Metodo di rilevamento:Colorimetric
- Tempo ai risultati:4 h 30 min
- Principio del dosaggio:Quantitative
- Durata a magazzino:Store for 6 months at 4 °C
- Range di rilevamento:1,25 - 80 ng/ml
- Temperatura di conservazione:4 °C
- Volume del campione:50 μl
- Sensibilità:0,75 ng/ml
- Stato regolamentazione:RUO
Specifications
About this item
Rat TSH beta ELISA kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of rat TSH beta in serum, plasma, tissue homogenates, and other biological fluids.
- Ready to use ELISA kit
- Detection range: 1,25 to 80 ng/ml
- Sensitivity: 0,75 ng/ml
- Sample type: Serum, plasma, tissue homogenates and other biological fluids
- Assay precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%
Rat TSH beta ELISA kit employs the competitive enzyme immunoassay technique for the quantitative measurement of rat TSH beta in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with TSH beta antigen. During the incubation, TSH beta present in the samples or standards competes with the fixed amount of immobilised TSH beta for binding sites on the Biotinylated Anti-TSH beta Antibody. The more TSH beta present in a sample or standard, the less Biotinylated Anti-TSH beta Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-TSH beta Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of TSH beta present in each sample or standard. The concentration of TSH beta can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.