Human HDL ELISA Kit

Human HDL ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human HDL in serum, plasma, tissue homogenates, and other biological fluids.

• Ready To Use ELISA kit
• Detection Range: 78,125 to 5000 ng/ml
• Sensitivity: 46,875 ng/ml
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Assay Precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%

Human HDL ELISA kit (A79420) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human HDL in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for HDL has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the HDL present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-HDL Antibody, which binds the captured HDL present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of HDL captured in each well. The concentration of HDL can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.

 : This is a ready to use ELISA kit that contains: Pre-coated 96 well microplate, lyophilised standard, sample dilution buffer, biotinylated detection antibody (100X), antibody dilution buffer, HRP-Streptavidin conjugate (SABC) (100X), SABC dilution buffer, TMB substrate, stop solution, wash buffer (25X), plate sealers, foil pouch, and protocol manual.