Specifications
- Conf:96 Tests
- Durata del test:Multiple steps
- Tipo di test:Sandwich
- Formato:Pre-coated
- Primary antibody reactivity:Mouse
- Target protein:beta 2 Microglobulin
- Descrizione:Mouse beta 2 microglobulin ELISA kit
- Tiipo del campione:Serum, plasma, tissue homogenates and other biological fluids
- Reattività incrociata:Mouse beta 2 Microglobulin ELISA Kit exhibits high specificity and excellent specificity for the detection of mouse beta 2 Microglobulin. No significant cross-reactivity or interference between beta 2 Microglobulin and analogues was observed.
- Metodo di rilevamento:Colorimetric
- Tempo ai risultati:4 h 30 min
- Principio del dosaggio:Quantitative
- Durata a magazzino:Store for 6 months at 4 °C
- Range di rilevamento:1,563 - 100 ng/ml
- Temperatura di conservazione:4 °C
- Volume del campione:100 μl
- Sensibilità:0,938 ng/ml
- Stato regolamentazione:RUO
Specifications
About this item
Mouse beta 2 Microglobulin ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse beta 2 Microglobulin in serum, plasma, tissue homogenates, and other biological fluids.
- Ready To Use ELISA kit
- Detection Range: 1,563 to 100 ng/ml
- Sensitivity: 0,938 ng/ml
- Sample Type: Serum, plasma, tissue homogenates and other biological fluids
- Assay Precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%
Mouse beta 2 Microglobulin ELISA kit (A79128) employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse beta 2 Microglobulin in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for beta 2 Microglobulin has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the beta 2 Microglobulin present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-beta 2 Microglobulin Antibody, which binds the captured beta 2 Microglobulin present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of beta 2 Microglobulin captured in each well. The concentration of beta 2 Microglobulin can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.