Specifications
- Conf:96 Tests
- Durata del test:Multiple steps
- Tipo di test:Sandwich
- Formato:Pre-coated
- Primary antibody reactivity:Human
- Target protein:Complement C3d
- Descrizione:Human complement C3d ELISA kit
- Tiipo del campione:Serum, plasma, tissue homogenates and other biological fluids
- Reattività incrociata:Human Complement C3d ELISA Kit exhibits high specificity and excellent specificity for the detection of human Complement C3d. No significant cross-reactivity or interference between Complement C3d and analogues was observed.
- Metodo di rilevamento:Colorimetric
- Tempo ai risultati:4 h 30 min
- Principio del dosaggio:Quantitative
- Durata a magazzino:Store for 6 months at 4 °C
- Range di rilevamento:7,813 - 500 ng/ml
- Temperatura di conservazione:4 °C
- Volume del campione:100 μl
- Sensibilità:4,688 ng/ml
- Stato regolamentazione:RUO
Specifications
About this item
Human Complement C3d ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Complement C3d in serum, plasma, tissue homogenates, and other biological fluids.
- Ready To Use ELISA Kit
- Detection Range: 7,813 to 500 ng/ml
- Sensitivity: 4,688 ng/ml
- Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay Precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%
Human Complement C3d ELISA Kit (A79152) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human Complement C3d in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for Complement C3d has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Complement C3d present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Complement C3d Antibody, which binds the captured Complement C3d present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Complement C3d captured in each well. The concentration of Complement C3d can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.