Specifications
- Conf:96 Tests
- Durata del test:Multiple steps
- Tipo di test:Sandwich
- Formato:Pre-coated
- Primary antibody reactivity:Rat
- Target protein:hnRNP L
- Descrizione:Rat hnRNP L ELISA kit
- Tiipo del campione:Serum, plasma, tissue homogenates, and other biological fluids
- Reattività incrociata:Rat hnRNP L ELISA kit exhibits high specificity and excellent specificity for the detection of rat hnRNP L. No significant cross-reactivity or interference between hnRNP L and analogues was observed.
- Metodo di rilevamento:Colorimetric
- Tempo ai risultati:4 h 30 min
- Principio del dosaggio:Quantitative
- Durata a magazzino:Store for 6 months at 4 °C
- Range di rilevamento:15,625 - 1000 pg/ml
- Temperatura di conservazione:4 °C
- Sensibilità:9,375 pg/ml
- Stato regolamentazione:RUO
Specifications
About this item
Rat hnRNP L ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rat hnRNP L in serum, plasma, tissue homogenates, and other biological fluids.
- Ready to use ELISA kit
- Detection range: 15,625 to 1000 pg/ml
- Sensitivity: 9,375 pg/ml
- Sample type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay precision: Intra assay: CV <8%, Inter assay: CV <10%
Rat hnRNP L ELISA kit (A303773) employs the sandwich enzyme immunoassay technique for the quantitative measurement of rat hnRNP L in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for hnRNP L has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the hnRNP L present in each sample is bound to the wells by the immobilised antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-hnRNP L antibody, which binds the captured hnRNP L present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of hnRNP L captured in each well. The concentration of hnRNP L can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.