Specifications
- Conf:96 Tests
- Durata del test:Multiple steps
- Tipo di test:Sandwich
- Formato:Pre-coated
- Primary antibody reactivity:Mouse
- Target protein:GJB2
- Descrizione:Mouse GJB2 ELISA kit
- Tiipo del campione:Serum, plasma, tissue homogenates and other biological fluids
- Reattività incrociata:Mouse GJB2 ELISA Kit exhibits high specificity and excellent specificity for the detection of mouse GJB2. No significant cross-reactivity or interference between GJB2 and analogues was observed.
- Metodo di rilevamento:Colorimetric
- Tempo ai risultati:4 h 30 min
- Principio del dosaggio:Quantitative
- Durata a magazzino:Store for 6 months at 4 °C
- Range di rilevamento:19,531 - 1250 pg/ml
- Temperatura di conservazione:4 °C
- Volume del campione:100 μl
- Sensibilità:11,719 pg/ml
- Stato regolamentazione:RUO
Specifications
About this item
Mouse GJB2 ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse GJB2 in serum, plasma, tissue homogenates, and other biological fluids.
- Ready to use ELISA kit
- Detection range: 19,531 to 1250 pg/ml
- Sensitivity: 11,719 pg/ml
- Sample type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay precision: Intra assay: CV <8%, Inter assay: CV <10%
Mouse GJB2 ELISA Kit (A76386) employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse GJB2 in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for GJB2 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the GJB2 present in each sample is bound to the wells by the immobilised antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-GJB2 Antibody, which binds the captured GJB2 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of GJB2 captured in each well. The concentration of GJB2 can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.