Specifications
- Conf:96 Tests
- Durata del test:Multiple steps
- Tipo di test:Sandwich
- Formato:Pre-coated
- Primary antibody reactivity:Mouse
- Target protein:Complement C1QA
- Descrizione:Mouse complement C1QA ELISA kit
- Tiipo del campione:Serum, plasma, tissue homogenates and other biological fluids
- Reattività incrociata:Mouse Complement C1QA ELISA kit exhibits high specificity and excellent specificity for the detection of mouse Complement C1QA. No significant cross-reactivity or interference between Complement C1QA and analogues was observed.
- Metodo di rilevamento:Colorimetric
- Tempo ai risultati:4 h 30 min
- Principio del dosaggio:Quantitative
- Durata a magazzino:Store for 6 months at 4 °C
- Range di rilevamento:0,234 - 15 ng/ml
- Temperatura di conservazione:4 °C
- Volume del campione:100 μl
- Sensibilità:0,141 ng/ml
- Stato regolamentazione:RUO
Specifications
About this item
Mouse Complement C1QA ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse Complement C1QA in serum, plasma, tissue homogenates, and other biological fluids.
- Ready to use ELISA kit
- Detection range: 0,234 to 15 ng/ml
- Sensitivity: 0,141 ng/ml
- Sample type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay Precision: Intra assay: CV <8%, Inter assay: CV <10%
Mouse Complement C1QA ELISA kit (A77771) employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse Complement C1QA in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for Complement C1QA has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Complement C1QA present in each sample is bound to the wells by the immobilised antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Complement C1QA Antibody, which binds the captured Complement C1QA present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Complement C1QA captured in each well. The concentration of Complement C1QA can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.