For separations of proteins and their variants using Thermo Scientific™ ProPac SCX and WCX strong and weak cation-exchange columns, which provide exceptionally high resolution.

Based on stationary phases composed of 10 µm nonporous polymeric beads, these columns resolve isoforms that differ by a single charged residue. A thin, hydrophilic layer grafted to the particle core eliminates unwanted secondary interactions. The cation-exchange surface provides pH-based selectivity control and fast mass transfer for high-efficiency separation and moderate capacity.