Specifiche

• Coniugazione:
  Non coniugato
• Protein/peptide type:
  Recombinant
• Sorgente luminosa:
  HEK293 cells
• Specie:
  Human
• Condizioni di conservazione:
  –20 °C
• Contenuto di endotossine:
  Less than 1,0 EU per μg by the LAL method.
• Attività biologica:
  Lyophilised from 0,22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH 7,5. Normally trehalose is added as protectant before lyophilisation. Measured by its binding ability in a functional ELISA. Immobilised human LAG-3, mouse IgG2a Fc Tag (Cat. No. ACRBLA3-H52Aa) at 2 μg/ml (100 μl/well) can bind anti-human LAG3 MAb, human IgG1 with a linear range of 1 - 10 ng/ml (QC tested). Measured by its binding ability in a functional ELISA. Immobilised human LAG-3, mouse IgG2a Fc Tag (Cat. No. ACRBLA3-H52Aa) at 10 μg/ml (100 μl/well) can bind cynomolgus / rhesus macaque FGL1, Fc Tag (Cat. No. ACRBFG1-C5269) with a linear range of 0,01 - 0,156 μg/ml (routinely tested). Flow cytometry assay shows that Human LAG3, Mouse IgG2a Fc Tag (Cat. No. ACRBLA3-H52Aa) can bind to daudi cell surface. The concentration of LAG3 used is 0,3 μg/ml (routinely tested). FACS analysis shows that the binding Human LAG3, mouse IgG2a Fc Tag (Cat. No. ACRBLA3-H52Aa) to daudi cells was inhibited by increasing concentration of neutralising Anti-LAG3 antibody. The concentration of LAG3 used is 1 μg/ml. The IC50 is 0,165 μg/ml (routinely tested).
• ID gene:
  P18627-1
• Senza corriere:
  Y
• Protease-free:
  Y
• Senza animali:
  Y
• Protein synonyms:
  FDC|CD223|LAG3
• Protein/peptide name:
  LAG-3
• Purezza:
  >95%
• Peso molecolare:
  73,3 kDa
• Livello di endotossine:
  Low
• Formulazione:
  Lyophilised from 0,22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH 7,5. Normally trehalose is added as protectant before lyophilisation.
• Esente da nucleasi:
  Y
• Temperatura di spedizione:
  Powder,RT