Technische Daten

• Coniugazione:
  Non coniugato
• Protein/peptide type:
  Recombinant
• Sorgente luminosa:
  HEK293 cells
• Specie:
  Human
• Condizioni di conservazione:
  –20 °C
• Contenuto di endotossine:
  Less than 0,1 EU per μg by the LAL method.
• Attività biologica:
  Lyophilised from 0,22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH 7,5. Normally trehalose is added as protectant before lyophilisation. Measured by its binding ability in a functional ELISA. Immobilised Human B7-1, Fc Tag (Cat. No. ACRBB71-H5259) at 2 μg/ml (100 μl/well) can bind Human CTLA-4, mouse IgG2a Fc tag, low endotoxin (Cat. No. ACRBCT4-H52A4) with a linear range of 0,1 - 2 ng/ml (QC tested). Measured by its binding ability in a functional ELISA. Immobilised Human B7-2, Fc Tag (Cat. No. ACRBCD6-H5257) at 2 μg/ml (100 μl/well) can bind Human CTLA-4, mouse IgG2a Fc tag, low endotoxin (Cat. No. ACRBCT4-H52A4) with a linear range of 0,5 - 7,8 ng/ml (Routinely tested). Measured by its binding ability in a functional ELISA. Immobilised Ipilimumab at 5 μg/ml (100 μl/well) can bind Human CTLA-4, mouse IgG2a Fc tag, low endotoxin (Cat. No. ACRBCT4-H52A4) with a linear range of 0,1 - 0,8 ng/ml (Routinely tested). Loaded Human CTLA-4, mouse IgG2a Fc tag, low endotoxin (Cat. No. ACRBCT4-H52A4) on Protein A Biosensor, can bind Human B7-1, His Tag (Cat. No. ACRBB71-H5228) with an affinity constant of 0,52 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). Loaded Human CTLA-4, mouse IgG2a Fc tag, low endotoxin (Cat. No. ACRBCT4-H52A4) on Protein A Biosensor, can bind Human B7-2, His Tag (Cat. No. ACRBCD6-H5223) with an affinity constant of 1,6 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
• ID gene:
  P16410-1
• Senza corriere:
  Y
• Protease-free:
  Y
• Senza animali:
  Y
• Protein synonyms:
  CTLA4|CD152
• Protein/peptide name:
  CTLA-4
• Purezza:
  >95%
• Peso molecolare:
  40,6 kDa
• Livello di endotossine:
  Low
• Formulazione:
  Lyophilised from 0,22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH 7,5. Normally trehalose is added as protectant before lyophilisation.
• Esente da nucleasi:
  Y
• Temperatura di spedizione:
  Powder,RT