Specifications

• Conf:
  100 µG
• Coniugazione:
  Non coniugato
• Protein/peptide type:
  Recombinant
• Sorgente luminosa:
  HEK293 cells
• Specie:
  Human
• Condizioni di conservazione:
  –20 °C
• Contenuto di endotossine:
  Less than 1,0 EU per μg by the LAL method.
• Attività biologica:
  Lyophilised from 0,22 μm filtered solution in PBS, pH 7,4. Normally trehalose is added as protectant before lyophilisation. Anti-Human BCMA MAb (human IgG1) captured on CM5 chip via Anti-Human IgG Fc antibodies surface, can bind Human BCMA, His Tag (Cat. No. BCA-H522y) with an affinity constant of 32,3 nM as determined in a SPR assay (Biacore T200) (Routinely tested). Anti-BCMA neutralising Antibody, Mouse IgG1 (Clone: AM43, Cat. No. BCA-M43) captured on CM5 chip via anti-mouse antibodies surface can bind Human BCMA, His Tag (Cat. No. BCA-H522y) with an affinity constant of 0,388 nM as determined in a SPR assay (Biacore T200) (Routinely tested). Loaded Anti-Human BCMA MAb (human IgG1) on Protein A Biosensor, can bind Human BCMA, His Tag (Cat. No. BCA-H522y) with an affinity constant of 35,8 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). Loaded Biotinylated Human BAFF, His,Avitag (active trimer) (MALS verified) (Cat. No. BAF-H82Q2) on SA Biosensor, can bind Human BCMA, His Tag (Cat. No. BCA-H522y) with an affinity constant of 1,1 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). Loaded Biotinylated Human BAFF, Avitag,Fc Tag (active trimer) (MALS verified) (Cat. No. BAF-H82F3) on SA Biosensor, can bind Human BCMA, His Tag (Cat. No. BCA-H522y) with an affinity constant of 1 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). 293 cells were transfected with anti-BCMA-scFv and RFP tag. 2e5 of the cells were first stained with B. Human BCMA, His Tag (Cat. No. BCA-H522y, 0,03 μg/ml) and C. His tag protein control, followed by FITC-conjugated anti-6xHis tag antibody. A. Non-transfected 293 cells and C. His tag protein control were used as negative control. RFP was used to evaluate CAR (anti-BCMA-scFv) expression and FITC was used to evaluate the binding activity of Human BCMA, His Tag (Cat. No. BCA-H522y).
• ID gene:
  Q02223-1
• Senza corriere:
  Y
• Protease-free:
  Y
• Senza animali:
  Y
• Protein synonyms:
  CD269|TNFRSF17|BCM
• Protein/peptide name:
  BCMA
• Purezza:
  >90%
• Peso molecolare:
  7,8 kDa
• Livello di endotossine:
  Low
• Formulazione:
  Lyophilised from 0,22 μm filtered solution in PBS, pH 7,4. Normally trehalose is added as protectant before lyophilisation.
• Esente da nucleasi:
  Y
• Temperatura di spedizione:
  Powder, RT