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Human recombinant OX40 Ligand (from HEK293)
 
Human recombinant OX40 Ligand (from HEK293)

 

  • Coniugazione:
    Non coniugato
  • Protein/peptide type:
    Recombinant
  • Sorgente luminosa:
    HEK293 cells
  • Specie:
    Human
  • Condizioni di conservazione:
    –20 °C
  • Contenuto di endotossine:
    Less than 0,1 EU per μg by the LAL method.
  • Attività biologica:
    Lyophilised from 0,22 μm filtered solution in PBS, pH 7,4, Normally trehalose is added as protectant before Lyophilisation,; Measured by its binding ability in a functional ELISA, Immobilised Human OX40, Fc Tag (Cat, No, OX0-H5255) at 0,5 μg/ml (100 μl/well) can bind Human OX40 Ligand, Mouse IgG2a Fc Tag, low endotoxin (Cat, No, OXL-H5250) with a linear range of 0,8-13 ng/ml (QC tested),; Loaded Human OX40 Ligand, Mouse IgG2a Fc Tag, low endotoxin (Cat, No, OXL-H5250) on Protein A Biosensor, can bind Human OX40, His Tag (Cat, No, OX0-H5224) with an affinity constant of 2 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested),; FACS assay shows that recombinant Human OX40 Ligand, Mouse IgG2a Fc Tag, low endotoxin (Cat, No, OXL-H5250) can bind to 293T cell overexpressing human OX40, The concentration of OX40 Ligand is 0,003 μg/ml (Routinely tested),; FACS analysis shows that the binding of Human OX40 Ligand, Mouse IgG2a Fc Tag, low endotoxin (Cat, No, OXL-H5250) to 293T overexpressing OX40 was inhibited by increasing concentration of neutralizing anti-OX40 antibody, The concentration of OX40 Ligand used is 0,002 μg/ml, IC50=3,021 μg/ml (Routinely tested).
  • ID gene:
    P23510-1
  • Senza corriere:
    Y
  • Protease-free:
    Y
  • Senza animali:
    Y
  • Protein synonyms:
    TNFSF4|CD134L|TXGP1|OX40L|Glycoprotein Gp34|CD134 ligand|CD252
  • Protein/peptide name:
    OX40 Ligand
  • Purezza:
    >95%
  • Peso molecolare:
    42,3 kDa
  • Livello di endotossine:
    Low
  • Formulazione:
    Lyophilised from 0,22 μm filtered solution in PBS, pH 7,4, Normally trehalose is added as protectant before Lyophilisation.
  • Esente da nucleasi:
    Y
  • Temperatura di spedizione:
    Powder,RT

 

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