SURE competent cells

SURE 2 competent cells are ideal for routine cloning of DNA with secondary structures.

Lack components of the pathways that catalyse the rearrangement and deletion of nonstandard secondary and tertiary structures
Allows for blue-white colour screening

Replicating eukaryotic DNA in prokaryotic cells can be problematic. Particular eukaryotic genes may contain inverted repeats or secondary structures, such as Z-DNA, that can be rearranged or deleted by E. coli DNA repair systems.

SURE competent cells are deficient in the E. coli genes involved in the rearrangement and deletion of DNA, thus improving cloning efficiencies of DNA containing irregular structures in prokaryotic cells. SURE cells are restriction minus (McrA-, McrCB-, McrF-, Mrr-, HsdR-) endonuclease (endA) deficient, and recombination (recB recJ) deficient.

: For research use only. Not for use in diagnostic procedures.

SURE 2 competent cells are ideal for routine cloning of DNA with secondary structures.

Lack components of the pathways that catalyse the rearrangement and deletion of nonstandard secondary and tertiary structures
Allows for blue-white colour screening

: For research use only. Not for use in diagnostic procedures.

Product Family Options

Descrizione
Caratteristiche
Pk Info
AGLS200227
SURE electroporation-competent cells
For cloning DNA with secondary structures, ≥1×10¹⁰ transformants/µg
5×0,1 ml

1 * 5 ST
1 * 5 ST - SFr. 454.00
SFr. 454.00
1 * 5 ST
SFr. 454.00
AGLS200238
SURE competent cells
For routine cloning of DNA with secondary structures, ≥5×10⁸ transformants/µg
5×0,2 ml

1 * 1 mL
1 * 1 mL - SFr. 433.00
SFr. 433.00
1 * 1 mL
SFr. 433.00
AGLS200152
SURE 2 supercompetent cells
High-efficiency derivative for cloning DNA with secondary structures, >1×10⁹ transformants/µg
10×0,1 ml

1 * 1 mL
1 * 1 mL - SFr. 442.00
SFr. 442.00
1 * 1 mL
SFr. 442.00