illustra™ triplePrep Kit

illustra™ triplePrep Kit è progettato per l'isolamento rapido e la purificazione ad alta resa di DNA genomico (gDNA), RNA totale e proteine denaturate totali da tessuti animali indivisi e cellule di mammiferi. Il flusso di lavoro semplificato riduce il numero totale di passaggi, consentendo la preparazione di tutti e tre gli analiti in meno di un'ora.

• High-quality genomic DNA with a greater proportion of higher molecular weight DNA and less shearing
• Demonstrated application to various types of whole blood including human, horse, rabbit, rat, and mouse
• Increased ease of use with reduced pipetting volume changes, one centrifugation setting, and color-coded kit components
• Verified in several downstream applications including real-time PCR, endpoint PCR, multiplex PCR, and restriction enzyme digests

The purification process involves minimal shearing resulting in the production of 5 to 10 μg of good quality, intact genomic DNA from a 200 μl sample. Input sample volumes can vary from 50 μl to 1 ml. The method uses a simple genomic DNA purification protocol that uses chaotropic agents to extract DNA from blood cells, denature protein components, and promote the selective binding of DNA to a column-based, novel silica membrane. The elution volume can be modified as necessary to obtain the appropriate genomic DNA concentration for most downstream molecular biological applications.

 : Kit contains lysis buffer type 15 (75 ml), wash buffer type 6 (12 ml), elution buffer type 5 (12 ml), elution buffer type 9 (7,5 ml), protein precipitation buffer type 1 (2×17,5 m)l, one vial DNase I, DNase reaction buffer type 1 (1,4 ml), illustra Mini columns (50X DNA), illustra Mini columns (50X RNA), collection tubes (200) and an instruction booklet.

 : Kit contains illustra blood genomicPrep spin columns prepacked with a novel silica membrane, lyophilised proteinase K powder, lysis solution, wash and elution buffers, microcentrifuge collection tubes, a full protocol booklet, and a detachable, quick reference protocol card.

 : Yields and purities of gDNA, total RNA, and total denatured proteins can vary with user and depend on the nature and condition of the input sample.