For the quantification of RvD1.

• Detect RvD1 in multiple sample types
• Assay 24 samples in triplicate or 36 samples in duplicate
• Measure RvD1 levels down to 15 pg/ml
• Incubation: 18 hours; Development: 90 – 120 minutes; Read: Colorimetric at 405 – 420 nm
• Midpoint: 50% B/B₀: 50 – 100 pg/ml

Resolvin D1 ELISA Kit is a competitive assay that can be used for quantification of RvD1. Due to the number and variation of potential sample types, this assay has been validated in Cayman’s ELISA Buffer (Item No. 400060) diluted to 1X (0.1 M potassium phosphate containing 0.1% BSA, 0.1% sodium chloride, 1 mM EDTA, and 0.01% sodium azide) and DMEM containing phenol red and 10% FBS.