Specifiche
- Tipo anticorpo:Primary
- Tipo di anticorpi:Ki-67
- Simbolo dell'antigene:KI67
- Clonalità:Monoclonale
- Clone:6B4
- Coniugazione:Non coniugato
- Ospite:Mouse
- ImmunoChimica:Yes
- ImmunoFluorescenza:Yes
- Isotipo:IgG1
- Reattività:Human
- Western blot:Yes
- Formato:Purified IgG
- Forma:Lyophilized
- Sinomini antigene:Proliferation marker protein Ki-67|Antigen KI-67|Antigen Ki67|Antigen identified by monoclonal antibody Ki-67
- UniProtKB:Uniprot # P46013
- Temperatura di conservazione:Store lyophilised antibody at 2-8 °C. After reconstitution divide into aliquots and store at –20 °C for long-term storage. Store at 2-8 °C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
- Temperatura di spedizione:Ambient
- Immunogeno:Recombinant human Ki-67 protein (amino acids 1,111-1,490) expressed in and purified from E. coli.
- Purificazione:Affinity purified
- Taglia:100 µg
- Conf:100 µG
Specifiche
Informazioni su questo articolo
Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable). Ref: uniprot.org
Western blotting (1:1,000-1:5,000) and Immunocytochemistry (1:2,000-1:5,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Type: Primary
Antigen: KI67
Clonality: Monoclonal
Clone: 6B4
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG1
Reactivity: Human