- Conf:10 µG
- Protein/peptide type:Recombinant
- Specie:Mouse
- Seq:02
- Ricostituzione:Reconstitute with 100 µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
- Protein/peptide name:TNC:TWEAK (soluble)
- Purezza:>95% (SDS-PAGE)
- Concentrazione:0,1 mg/ml after reconstitution
- Formulazione:Lyophilised. Contains PBS.
Produced in HEK 293 cells. The extracellular domain of mouse TWEAK (aa 94-249) is fused at the N-terminus to tenascin-C (TNC) (chicken) (aa 110-139) and a FLAG®-tag.
Some TNF ligands (such as TWEAK (mouse) or FasL (mouse)) produced as soluble forms do not assemble into stable trimers and show low receptor binding.
Addition of the tenascin-C oligomerization domain (TNC) (from chicken) at the N-terminus of recombinant mouse soluble TWEAK stabilizes the TNF homology domain (THD) of rmsTWEAK into a trimer and increases binding to its respective TNF receptor.
Note: For some TNF ligands, such as FasL (mouse), activated by further oligomerization (> hexamer), the presence of TNC is not sufficient and ligand crosslinking using the 'Enhancer for Ligands' (Prod. No. ALX-804-034) is required.