Auto-Ubiquitinylation Kit

This E3 ligase auto-ubiquitinylation kit enables proteins to be tested for ubiquitin E3 ligase activity through assessment of their ability to under auto-ubiquitinylation. Utilizing the first three steps in the ubiquitin cascade the kit facilitates ubiquitinylation of known or putative E3 ligase enzymes followed by Western blot analysis using the highly sensitive reagents provided or using antibodies to the specific protein of interest (user supplied). A high integrity ubiquitin E3 ligase enzyme is also provided for use as a positive control.

 : Kit contains 20X Ubiquitin Activating Enzyme Solution (E1) (25µL), 20X Ubiquitin Conjugating Enzyme Solution (E2) (25µL), 10X Ubiquitin Solution (Ub) (25µL), 20X Control Ubiquitin Ligase Solution (E3) (25µL), 20X Mg-ATP Solution (25µL), 10X Ub E3 ligase Buffer (50µL), Ubiquitin antibody solution (25µL).