M1 and M 2 macrophages are derived from purified CD14⁺ monocytes. CD14⁺ monocytes were positively selected via immunomagnetic beads and were cultured in the presence of GM-CSF(M1) or M-CSF(M2) for 10 days. The phenotyping was confirmed by morphology and the expression of specific biomarkers.

• Purity >90% by flow cytometry
• Test negative for Hepatitis B and C, HIV 1/2, HTLV-I/II, HIV-1/HCV/HBV Nucleic Acid, WNV Nucleic Acid, Trypanasoma cruzi and Syphilis
• Cryopreserved in CryoStor® CS10 media

Peripheral blood cells are collected from normal human volunteers by direct collection or by using the COBE® Spectra or Spectra Optia® Apheresis System where cell-rich leukapheresis product is extracted using continuous flow centrifugal technology directly into the collection bag.

Macrophages are a type of white blood cell found in all tissues patrolling for potential pathogens. Macrophages are key players in the immune response, basically ridding the body of worn-out cells, foreign substances, microbes, and cancer cells and alerting the immune system to the presence of invaders. Because macrophages possess the ability to eliminate pathogens and recruit and instruct other immune cells, they play a central role in protecting the body and contribute to the pathogenesis of inflammatory and degenerative diseases.

Macrophages can modify themselves and change their functional phenotype depending on the environmental cues: M1 macrophages encourage inflammation. M2 macrophages decrease inflammation and encourage tissue repair.

Storage: Either prepare cells for long term storage in liquid nitrogen vapour phase or thaw for use. Storage in liquid phase nitrogen is NOT recommended. Short term storage of cells (less than 2 weeks) at –80°C is acceptable, but should be minimised to ensure maximum stability. Once thawed, samples must be used immediately.