Specifications
- Conf:50 µG
- Tipo di enzima:Recombinant
- Sorgente luminosa:E. coli
- Specie:H. influenzae
- Condizioni di conservazione:Stable for at least 6 months after receipt when stored at –80 °C.
- Nome dell'enzima:NAD Nucleotidase
- Attività enzimatica:With AMP: 856.4 nmol of Ade/min/mg of protein
With NMN: 110,3 nmol of NR/min/mg of protein
With NAD: 5.4 nmol of Ade/min per mg of protein | 5.3 nmol of NR/min/mg of protein - Purezza:>98% (SDS-PAGE)
- Sequenza:Full-length NAD 5’-nucleotidase without the signal peptide corresponding to the first 26 aa
- Formulazione:Liquid, In 5 mM KH₂PO₄, pH 6.8 containing 100 mM TRIS-HCl (pH 8.5) and 10 mM MgCl₂.
Specifications
About this item
NadN (NAD nucleotidase) is a periplasmic enzyme from Haemophilus influenzae, a major pathogen of the respiratory tract in humans that has developed the capability to exploit host NAD(P) for its nicotinamide dinucleotide requirement. NadN plays a central role by degrading NAD into adenosine and NR (nicotinamide riboside), NMN to nicotinamide riboside and AMP to adenosine. It shows a broad substrate specificity, recognising either mono- or di-nucleotide nicotinamides and different adenosine phosphates with a maximal activity on 5'-adenosine monophosphate. It can be used as an economically alternative to produce NR (nicotinamide riboside). Catalytic activity: NAD=AMP + NMN=Ade+NR+PPi | AMP=Ade+Pi | NMN=NR+Pi.
Fusion-Tag: Tag Free
This recombinant proteins is for research use only.