Protease isolated from S. aureus, strain V8, this protease exhibits cleavage of peptide bonds on the COOH-terminal side of either aspartate or glutamate residues.

Unit definition: one unit will hydrolyse casein to change A280 0,001 per minute, pH 7,8, at 37 °C. Lyophilised activity: Approx. 500 units/mg Ref.: 1. Drapeau, G.A.,Boily, Y., Houmard, J., Biol. Chem., 217: 6720 (1972). 2. Kunitz,M., J. Gen. Physiol., 30: 291 (1947).

pH: 4,0 and 7,8 with hemoglobin substrate (optimum pH)