Specifications

Conf:
20 µl
Sorgente luminosa:
E. coli carrying the Klenow (3'-5' exo-) gene
Seq:
02
Condizioni di conservazione:
Stable for at least 1 year after receipt when stored at –20 °C
Forma:
Liquid
Condizioni di spedizione:
Dry Ice
Nome dell'enzima:
Klenow (3'-5' exo-)
Attività enzimatica:
10000 U/mg
Purezza:
≥99% (SDS-PAGE)
Formulazione:
In 20 mM TRIS-HCl, pH 7,5, containing 50% glycerol, 0,1 mM EDTA and 1 mM DTT.

Specifications

About this item

Klenow (3′-5′ exo-) is a mesophilic DNA polymerase deficient in both proofreading (3′-5′) and nick-translation (5′-3′) nuclease activities, and that displays a moderate strand displacement activity during DNA synthesis. The protein is expressed as a truncated product of the E.coli PolA gene and contains the D355A and E357A mutations.

Used for DNA labeling
Not for 5´→3´ or 3´→5´exonuclease activity
N-terminal truncated DNA Polymerase I which retains polymerase activity

It is formulated of liquid in 20 mM TRIS-HCl, pH 7,5, containing 50% glycerol, 0,1 mM EDTA and 1 mM DTT.