Alkaline phosphatase is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids.

• Enzyme Commission Number: E.C. 3.1.3.1
• Isoelectric point (pI): 5,7 (Lit.)

It is used for removing phosphate monoester to prevent self ligation. It can be also be used as a label for enzyme immunoassays.

It is fully active in the presence of sodium azide (0,05%) and Triton X-100 (0,1%).

Activators: Zn, Mg²⁺, Ca²⁺, Tris buffer, diethanolamine
Inhibitors: Acidification to pH 4,5 reversibly inactives the enzyme; inorganic phosphate, chelating agents such as EDTA

Unit definition: One unit liberates one µmole of p-nitrophenol per minute at 37 °C, pH 10,15 in Tris buffer. The enzyme activity is determined by measuring the increase in optical density at 405 nm resulting from the hydrolysis of p-nitrophenyl phosphate.