Neuraminidase of non-pathogenic streptococci, is prepared from the culture filtrate by Ammonium Sulfate precipitation and Ion-exchange chromatography.

• Enzyme Commission Number: E.C.3.2.1.18
• Activators: Mg²⁺, Co²⁺, Ca²⁺, and K⁺ (10mM), these activators accelerate activity 10 to 20%
• Inhibitors: Ag⁺, Hg⁺, Cu²⁺, and Potassium Permanganate (10mM)
• Isoelectric point (pI): 7,7 ±0,1

Unit definition: One unit causes the hydrolysis of one µmole of sialyllactose per minute at pH 6,5, 37 °C.