Specifications
- Tipo anticorpo:Primary
- Tipo di anticorpi:Dynamin 1
- Simbolo dell'antigene:DNM1
- Clonalità:Policlonale
- Coniugazione:Non coniugato
- Ospite:Rabbit
- ImmunoChimica:Yes
- Isotipo:IgG
- Reattività:Human,Rat,Mouse
- Western blot:Yes
- Forma:liquid
- ID gene:1759
- Sinomini antigene:EC 3.6.5.5|DNM1|Dynamin BREDNM19|DNM|brain|dynamin-1|Dynamin|B-dynamin|D100
- Tampone di conservazione:Aqueous buffered solution containing 100ug/ml BSA, 50% glycerol and 0,09% sodium azide. Store at -20°C for 12 months.
- Concentrazione:1 ug/ul
- Temperatura di spedizione:4 °C
- Purificazione:Purified by Protein A.
- Taglia:100 μg
- Conf:100 µl
Specifications
About this item
Dynamin I is a GTPase enzyme required for the retrieval of synaptic vesicles after exocytosis and functions in endocytosis by stimulating assembly of invaginating synaptic vesicles (1). Dynamin I is phosphorylated in nerve terminals exclusively in the cytosolic compartment and in vitro by protein kinase C (PKC) (2–5). The phosphorylation site in PKC-phosphorylated Dynamin I is a single site at Serine 795, which is located near a binding site for the SH3 domain of p85, the regulatory subunit of phosphatidylinositol 3-kinase (2–5). Dephosphorylation is required for synaptic vesicle retrieval, suggesting that phosphorylation affects the subcellular localization of Dynamin I (5). Mouse, rat and human Dynamin I are phosphorylated on serine residues, including Ser 778, by Cdk5, regulating PACSIN1 recruitment and enabling synaptic vesicle endocytosis.
WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
Type: Primary
Antigen: DNM1
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype: IgG
Reactivity: Human, Mouse, Rat