The phenol-free total RNA purification kit provides a rapid method for the isolation and purification of total RNA from cultured animal cells, tissue samples, blood, bacteria, yeast, fungi, plants and viruses.

• Eliminate phenol and chloroform from RNA purification
• Enhance recovery of total RNA, including microRNA species
• Save time with a simple 20 minute protocol

The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other cellular components, such as proteins.

The purified RNA, of the highest integrity can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.