The Cdc25 phosphatases are key regulators of normal cell division and the cell's response to DNA damage. Cdc25 isoforms are dual-specificity phosphatases that remove inhibitory phosphates from Thr14 and Tyr15 in Cdk1 and Cdk2. Cdk1 is necessary for mitotic onset and shares an overlapping role with Cdk2 in controlling S phase initiation. Cdc25 isoforms are also key targets of the Chk1 and Chk2 checkpoint kinases, which inactivate Cdc25 to halt cell cycle progression when DNA is damaged or incompletely replicated. Mammalian cells have three Cdc25 isoforms: Cdc25A, Cdc25B and Cdc25C. Cdc25A, which regulates both early and late cell-cycle transitions, controls progression through S phase and entry into mitosis, whereas Cdc25B and Cdc25C primarily control entry into mitosis.

Western Blot: 1:2,000;

Type: Primary
Antigen: CDC25A (cell division cycle 25A)
Clonality: Monoclonal
Clone: PK1-AF2D12
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG1 Kappa
Reactivity: Human