Solubilisation buffer, 2D-Xtract™

A protein solubilisation buffer for 2D analysis must solubilise proteins effectively, without disturbing the native charge of the proteins. Urea based solubilisation buffers solubilise proteins effectively, however can modify the native charge of the proteins, due to carbamylation. Urea exists in equilibrium with ammonium cyanate that modifies α- and ε-amino groups, inducing changes in the isoelectric point of proteins leading to artifactual results.

• Convenient and simple to use extraction buffer
• No preparation required, simply hydrate and use
• Prevents urea induced protein carbamylation
• Prevents waste of unused reagents
• No artifactual protein bands due to dust and human skin contamination

2D-Xtract™ is a dry urea based pre-mixed and ready-to-use solubilisation buffer. Simply add an appropriate volume of the supplied rehydration buffer to the dry buffer and then use to solubilise proteins, saving time and improving the quality of IEF/2D gel electrophoresis. 2D-Xtract™ has optimised concentrations of urea, thiourea, CHAPS and non detergent sulfobetaine (ND SB) 201. 2D-Xtract™ is also designed to be used as a rehydration buffer for IPG strips.