Supplier: Charles River Laboratories Cell Solutions

A Leukopak is an enriched leukapheresis product collected from normal peripheral blood. It is composed of a variety of blood cells including monocytes, lymphocytes, platelets, plasma, and red cells. A leukopak contains a higher concentration of cells as compared to standard venipuncture collection methods or buffy coat products.

Supplier: Charles River Laboratories Cell Solutions

Human peripheral blood CD14⁺ monocytes are isolated from peripheral blood mononuclear cells (PBMCs) by either positive or negative selection using immunomagnetic cell separation procedures. Peripheral blood cells are collected from normal human volunteers by direct collection or by using the COBE® Spectra or Spectra Optia® Apheresis System where cell-rich leukapheresis product is extracted using continuous flow centrifugal technology directly into the collection bag.

Supplier: Charles River Laboratories Cell Solutions

Naive T cells are isolated by depleting CD45RO^₊ cells via immunomagnetic bead separation from a freshly collected leukopheresis product. Naive T cells are phenotypically classified as CD45⁺, CD^₊, and CD45RA^₊.

Supplier: Charles River Laboratories Cell Solutions

CD8⁺ cytotoxic T cells are isolated from mononuclear cells by positive selection using immunomagnetic cell separation procedures.

Supplier: Charles River Laboratories Cell Solutions

CD4⁺/CD25⁺ regulatory T cells were isolated from leukapheresis product by CD127 depletion and positive selection using CD25 immunomagnetic beads. Peripheral blood cells are collected from normal human volunteers by direct collection or by using the COBE® Spectra or Spectra Optia® Apheresis System where cell-rich leukapheresis product is extracted using continuous flow centrifugal technology directly into the collection bag.

Supplier: Charles River Laboratories Cell Solutions

Human mononuclear cells are isolated from IRB-consented volunteer donors diagnosed with HIV or AIDS.

Supplier: Charles River Laboratories Cell Solutions

Mononuclear cells used for researching the causes, diagnosis, supportive care and treatment of Diabetes Mellitus 2 (DM2), the most common form of diabetes. Human mononuclear cells are collected from human volunteer donors diagnosed with DM2 under IRB approved informed consent by whole blood or leukapheresis using the COBE® Spectra Apheresis System MNC collection protocol.

Supplier: G-Biosciences

GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.

Supplier: Novus Biologicals

p38 Overexpression Lysate (Adult Normal)

Supplier: ProSci Inc.

Ovary tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH 7,9, 1,5 mM MgCl₂, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors).

Supplier: PROVITRO

HFIB-d - Human Fibroblast from dermis - are obtained from human tissue, in particularly from the dermis, initially isolated in their in vivo state. The cells are then transitioned into a primary cell culture and subsequently aliquoted and frozen for preservation. It's crucial to note that HFIB cultures undergo careful methods during isolation, ensuring that they remain untransformed and unmutated, maintaining characteristics reflective of their in vivo state. These primary cultures have a limited in-vitro lifespan.

Supplier: PROVITRO

CD34⁺ stem and progenitor cells from umbilical cord blood are crucial for haematopoiesis and modulation of the immune response. These unique cells contribute to the formation of various blood cells, including immune effectors such as natural killer cells and cytotoxic T-cells, shaping both the adaptive and functional aspects of the immune system.

Supplier: PROVITRO

CD14⁺ T cells, obtained from mononuclear cells, are isolated through either negative or positive selection using immunogenetic cell separation techniques. These cells play a crucial role in the immune response against infections and are valuable for the investigation and diagnosis of immune reactions, particularly in infections and inflammation.

Supplier: ProSci Inc.

Liver tissue lysate (7 Days Old) was prepared by homogenisation in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7,4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0,1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin.

Supplier: ProSci Inc.

Skin tissue lysate (14 Days Old) was prepared by homogenisation in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7,4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0,1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin.

Supplier: Charles River Laboratories Cell Solutions

M1 and M 2 macrophages are derived from purified CD14⁺ monocytes. CD14⁺ monocytes were positively selected via immunomagnetic beads and were cultured in the presence of GM-CSF(M1) or M-CSF(M2) for 10 days. The phenotyping was confirmed by morphology and the expression of specific biomarkers.

Supplier: Charles River Laboratories Cell Solutions

CD19⁺ B cells are isolated from mononuclear cells by positive or negative selection using immunomagnetic cell separation procedures. Peripheral blood cells are collected from normal human volunteers by direct collection or by using the COBE® Spectra or Spectra Optia® Apheresis System where cell-rich leukapheresis product is extracted using continuous flow centrifugal technology directly into the collection bag.

Supplier: Charles River Laboratories Cell Solutions

Naive CD4⁺ T cells are isolated from mononuclear cells by positive selection using immunomagnetic cell separation procedures.

Supplier: Charles River Laboratories Cell Solutions

Peripheral blood mononuclear cells (PBMC) are isolated from blood collected in FDA-registered collection centres, from IRB consented healthy human donors and following cGMP and cGTP collection guidelines.

Supplier: Charles River Laboratories Cell Solutions

A Leukopak is an enriched leukapheresis product collected from normal peripheral blood. It is composed of a variety of blood cells including monocytes, lymphocytes, platelets, plasma, and red cells. A leukopak contains a higher concentration of cells as compared to standard venipuncture collection methods or buffy coat products.

Supplier: G-Biosciences

GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.

Supplier: G-Biosciences

GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.

Supplier: G-Biosciences

GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.

Supplier: PROVITRO

Isolated T cells, including CD3⁺ pan-T cells, CD4⁺ and CD8⁺ subgroups, have been obtained through immunomagnetic cell separation techniques employing positive or negative selection from mononuclear cells. These differentiated procedures ensure the precise isolation of the intended T-cell groups. CD4⁺ T cells play a role in coordinating immune responses, while CD8⁺ T cells exert a cytotoxic function.

Supplier: PROVITRO

Isolated T cells, including CD3⁺ pan-T cells, CD4⁺ and CD8⁺ subgroups, have been obtained through immunomagnetic cell separation techniques employing positive or negative selection from mononuclear cells.

Supplier: PROVITRO

B cells have been isolated from mononuclear cells through either positive or negative selection using immunomagnetic cell separation techniques.

Supplier: Novus Biologicals

PLAC1 Overexpression Lysate (Adult Normal)

Supplier: Novus Biologicals

HIF-2 alpha overexpression lysate (adult normal).

Supplier: Charles River Laboratories Cell Solutions

The mononuclear cell is a widely used product in research and clinical applications such as in microbiology, virology, oncology, vaccine development, transplant and regenerative biology, and toxicology.

Supplier: Charles River Laboratories Cell Solutions

CD56⁺ NK cells are isolated from mononuclear cells either by positive selection using an antibody that targets the CD56 antigen, or by negative selection using immunomagnetic cell separation procedures.