Hai cercato: Test PCR

Supplier: BIOPREMIER

SUPREME Real time detection uses real-time PCR for the detection of DNA present in the mitochondrial genome in a simple, reliable, and rapid procedure. The assay is based on 5’ nuclease real time PCR reactions to amplify a unique genomic sequence in the target.

Supplier: BIOPREMIER

Gluten detection test kit by using qPCR. Celiac disease is an autoimmune disorder associated with an intolerance to gluten proteins mainly present in wheat, barley, and rye.

Supplier: PRIMERDESIGN

PCR kits for the detection and simultaneous quantification of gastrointestinal pathogens.

Supplier: PRIMERDESIGN

PCR kits for the detection and simultaneous quantification of hepatitis viruses.

Supplier: PRIMERDESIGN

PCR kits for the detection and simultaneous quantification of human herpes viruses.

Supplier: Sartorius

The Microsart® ATMP Mycoplasma detection kit is especially designed for an easy and fast detection of mycoplasma in cell-based therapeutics like autologous chondrocyte transplants (ATMPs –advanced therapy medical products). Due to the short shelf life of these therapeutics, a fast result is needed. The PCR-technology makes it possible to reduce the time-to-result to only 3 hours.

Supplier: PRIMERDESIGN

Papillomaviruses are a diverse group of DNA-based viruses that infect the skin and mucous membranes of humans and a variety of animals. Although more than 100 different human papillomavirus (HPV) types have been characterised HPV 6, HPV 11, HPV-16 and HPV 18 are the most clinically relevant.

Supplier: BIOPREMIER

These kits allow the detection of allergic substances. Ideal for controlling and monitoring the various steps of food production.

Supplier: Sartorius

The Microsart® RESEARCH Mycoplasma real-time PCR kit is especially designed for fast and reliable Mycoplasma contamination control in cell cultures, cell culture supernatants and media most applicable in research and development, e.g. biotech and biopharmaceutical research and development, universities and governmental research groups or for in-process control during biopharmaceutical production processes.

Supplier: BIOPREMIER

I kit per la sicurezza degli alimenti forniscono una procedura semplice, affidabile e rapida per rilevare la presenza di uno specifico batterio patogeno. L'analisi è basata sulle reazioni in tempo reale di PCR della 5' nucleasi per amplificare la sequenza genomica univoca nel microrganismo bersaglio. La PCR è un metodo per amplificare una specifica sequenza del DNA in cui l'amplificazione avviene generalmente in una reazione comprendente una DNA polimerasi termostabile, nucleotidi e primer complementari alla sequenza bersaglio. Quando la soluzione viene riscaldata, la molecola di DNA viene denaturata e si separa in due filamenti. Con il raffreddamento della soluzione, i primer si legano (annealing) alle sequenze bersaglio nei filamenti di DNA separati e la DNA polimerasi sintetizza un nuovo filamento estendendo i primer con nucleotidi, creando una copia della sequenza del DNA (amplicon). Il ripetersi di questo ciclo di denaturazione, annealing ed estensione aumenta in maniera esponenziale il numero di amplicon bersaglio. Nella PCR in tempo reale vengono impiegate specifiche sonde a fluorescenza per rilevare il DNA amplificato mediante ibridizzazione con amplicon. Queste sonde sono collegate a un fluoroforo su una estremità e a uno smorzatore che sopprime la fluorescenza sull'altra. Se la sequenza bersaglio è presente durante la PCR, si verifica l'amplificazione e la sonda viene degradata, producendo un aumento della fluorescenza. La fluorescenza viene misurata da un rilevatore e il software associato ne traccia l'intensità a fronte del numero di cicli, consentendo di determinare la presenza o assenza dell'organismo bersaglio.

Supplier: BIOPREMIER

I kit per la sicurezza degli alimenti forniscono una procedura semplice, affidabile e rapida per rilevare la presenza delle specie animali nella carne e nei prodotti a base di carne. L'analisi è basata sulle reazioni in tempo reale di PCR della 5' nucleasi per amplificare la sequenza genomica univoca nell'organismo bersaglio. Ai sensi della direttiva 2002/86/CE della Commissione europea, è obbligatorio dichiarare gli ingredienti degli alimenti. In caso di carne e prodotti a base di carne, è necessario specificare sull'etichetta le specie animali. Inoltre, l'identificazione delle specie può essere altamente rilevante per i consumatori per motivi economici, medici, culturali e religiosi. La sostituzione fraudolenta di carni più economiche al posto di specie più costose, l'inclusione di carne in prodotti privi di carne (per vegetariani) e la presenza di allergeni nei prodotti alimentari sono chiari esempi dell'importanza di tale questione (Ballin, 2010; Dooley et al., 2004). Vari metodi si basano sull'identificazione delle proteine mediante elettroforesi e/o test immunologici. Tuttavia, tali metodi non sono affidabili per l'applicazione in prodotti altamente lavorati e riscaldati, a causa del deterioramento delle proteine. Il DNA è più stabile delle proteine durante la lavorazione e, per quanto possa essere frammentato da vari processi, le moderne metodologie per il DNA, come le tecniche basate su PCR, consentono comunque di identificare il DNA delle varie specie presenti in un campione (Lockley & Bardsley, 2000). Le tecniche di PCR in tempo reale sono particolarmente adatte per questi prodotti, dal momento che consentono di amplificare e identificare con elevata sensibilità e specificità anche piccoli frammenti di DNA (Dooley et al., 2004).

Supplier: Q BIOANALYTIC

Food safety experts like the German DGHM recommend to test warm water fish for vibrio cholerae . With this Real-Time PCR test you have a very versatile method for food testing, which can gives you results within 24 hrs. The test was developed and validated within the German Network for Vibrio Research 'VibrioNet' with many scientific institutions involved. It can be applied for food and sea water samples. In case of positive results you may be interested to determine whether or not the present strain has the ability to produce toxins. You can use our Real-Time PCR Test for toxicity gene of Vibrio cholera for this purpose VWR catalogue number: QBIAQB-RTI-19. You can use this test in combination with an appropriate pre- enrichment in alkaline peptone-water or you can confirm bacterial colonies on agar plates.

Supplier: Q BIOANALYTIC

Vibrio parahaemolyticus tox gene (tdh/trh): Food safety experts like the German DGHM recommend to test warm water fish for Vibrio parahaemolyticus. With this Real-Time PCR test you have a very versatile method for testing whether or not the present strain has the ability to produce toxins (tdh/trh). You can use our Real-Time PCR Test for Vibrio parahaemolyticus for testing for the presence of the species before. You can use this test in combination with an appropriate pre- enrichment in alkaline peptone-water or you can confirm bacterial colonies on agar plates.

Supplier: Q BIOANALYTIC

Legionella spec. is tested usually with cultural methods. However, this may take up to 14 days to deliver results and some strains are living but are not culturable. Using this Real-Time PCR test you may have results already after a few hours. You can use this test in combination with an appropriate pre- enrichment or you can do a DNA purification directly from the filter without pre-enrichment. The kit is also suitable to confirm bacterial colonies on agar plates.

Supplier: Q BIOANALYTIC

Testing for pathogenic E. coli like EHEC, EPEC , EIEC etc. can help to prevent foodborne infections as it has taken place in 2011 with E. coli strain O104 in Germany and with strain O157 at other places in the world. The real-time PCR kit allows the detection of stx1, stx2 and the eaeA gene which covers all different strains of pathogenic E. coli. The test can also be used for the confirmation of bacterial colonies.

Supplier: Q BIOANALYTIC

Kit for Vibrio cholerae tox gene (ctx). Food safety experts like the German DGHM recommend to test warm water fish for vibrio cholerae. With this Real-Time PCR test you have a very versatile method for testing whether or not the present strain has the ability to produce toxins. You can use our Real-Time PCR Test for Vibrio cholerae for testing for the presence of the species before. You can use this test in combination with an appropriate pre- enrichment in alkaline peptone-water or you can confirm bacterial colonies on agar plates.

Supplier: Q BIOANALYTIC

Vibrio alginolyticus causes eye, ear and wound infections. It can be tested using this Real-Time PCR test. The analysis can be done in water and food samples. You can use this test in combination with an appropriate pre- enrichment in alkaline peptone-water or you can confirm bacterial colonies on agar plates.

Supplier: Q BIOANALYTIC

OneCup it‘s so easy! The OneCup assay comes along with a minimum of pipetting steps. Everything is included in the mix. You only need to combine 20 μl of the mix with 2 μl of your DNA sample and everything is ready. You have a minimum of hands-on time and avoid mistakes during pipetting. You can run the test on all common block cyclers. Application: Detection of Salmonella in food samples and environmental samples subsequent to a pre-enrichment step according to DIN EN ISO 20837 and 20838. In combination with an appropriate pre-enrichment and DNA preparation the test complies with the official method of the German food and feed law §64. ASU L00.00-98. Test principle: The test is dedicated to detect DNA of Salmonella in a sample using Real-Time PCR in compliance with the ISO method mentioned below. The kit uses the TaqMan® principle. Thus the requirements of the ISO standard, that an amplification product has to be confirmed by a hybridization step, is fulfilled. Each reactions contains an internal amplification control. Therefore, false negative results due to inhibition of the reaction can be excluded. The test included the UNG enzyme to prevent re-amplification of contaminating amplicons.

Supplier: Q BIOANALYTIC

OneCup it‘s so easy! The OneCup assay comes along with a minimum of pipetting steps. Everything is included in the mix. You only need to combine 20μl of the mix with 2 μl of your DNA sample and everything is ready. You have a minimum of hands-on time and you avoid mistakes during pipetting. You can run the test on all common block cyclers. Application: Detection of Listeria monocytogenes in food samples and environmental samples subsequent to a pre-enrichment step according to DIN EN ISO 20837 and 20838.Test principle. The test is dedicated to detect DNA of Listeria monocytogenes in a sample using Real-Time PCR in compliance with the ISO method mentioned below. The kit uses the TaqMan® principle. Thus the requirements of the standard, that an amplification product has to be confirmed by a hybridization step, is fulfilled. Each reactions contains an internal amplification control. Therefore, false negative results due to inhibition of the reaction can be excluded. The test included the UNG enzyme to prevent re-amplification of contaminating amplicons.

Supplier: Q BIOANALYTIC

The Real-Time PCR screening kit is addressing three different targets: 35S promoter; NOS Terminator; CTP2-CP4 EPSPS. The tests are all in accordance with the JRC guideline. Advantage of the CTP2-CP4 EPSPS compared to FMV detection is that the used target includes artificial sequences, which are not present naturally like the virus sequence of FMV.

Supplier: Q BIOANALYTIC

Testing for pathogenic E. coli like EHEC, EPEC , EIEC etc. can help to prevent foodborne infections as it has taken place in 2011 with E. coli strain O104 in Germany and with strain O157 at other places in the world. The real-time PCR kit allows the detection of stx1, stx2 and the eaeA gene which covers all different strains of pathogenic E. coli. It also includes a test for Shigella. The test can also be used for the confirmation of bacterial colonies.

Supplier: Q BIOANALYTIC

Successful PCR applications depend on well purified DNA. In diagnostics and analytics of food and medical samples PCR and real-time PCR has many advantages over conventional methods. In all cases it is of importance to have well purified DNA for subsequent PCR, real-time PCR, hybridization or sequencing purposes. Purified DNA leads to higher sensitivity and also specificity of PCR based tests in difficult matrices. With QuickBlue Extraction and Purification Kit you always get high DNA yields and highly purified DNA. The kit uses paramagnetic nano-particles for purification. It can help to purify DNA from difficult matrices like food or even blood in soil for applications in forensic sciences. DNA in Seawater can also be purified. This method avoids extensive centrifugation steps and can thus be applied in environments where centrifuges are not usable. The kits includes proteinase K as a reagent for cell disruption, so that extraction and purification can be performed with the kit. DNA yield from 1 ml bacterial enrichment is 10 μg of Genomic DNA. The protocol can be adapted to automated magnetic bead separation instruments.

Supplier: Q BIOANALYTIC

Food safety experts like the German DGHM recommend to test warm water fish for Vibrio parahaemolyticus. With this Real-Time PCR test you have a very versatile method for food testing, which can gives you results within 24 hrs. The test was developed and validated within the German Network for Vibrio Research “VibrioNet” with many scientific institutions involved. It can be applied for food and sea water samples. In case of positive results you may be interested to determine whether or not the present strain has the ability to produce toxins (tdh/trh). You can use our Real-Time PCR Test for toxicity gene of Vibrio cholera for this purpose VWR catalogue number: QBIAQB-RTI-50. You can use this test in combination with an appropriate pre- enrichment in alkaline peptone-water or you can confirm bacterial colonies on agar plates.

Supplier: Q BIOANALYTIC

La gamma di prodotti RealQuick è stata ottimizzata per la PCR Real-Time e utilizza la tecnologia della sonda Taqman® per il rilevamento dei batteri patogeni umani. I kit sono composti in modo da poterli utilizzare in un'unica esecuzione.

Supplier: Q BIOANALYTIC

Vibrio vulnificus causes severe wound infections and is harmful when ingested. 33% of patients being infected die. The detection in food and water samples using this Real-Time PCR Test is very rapid and versatile. You have results available already after 24 hours. You can use this test in combination with an appropriate pre- enrichment in alkaline peptone-water or you can confirm bacterial colonies on agar plates.

Supplier: Q BIOANALYTIC

Cronobacter sakazakii (formerly Enterobacter sakazakii) is found naturally in the environment and can survive in dry conditions. It occurs in dry foods, such as powdered infant formula, milk powder and starches as well as in teas. Especially young infants are at high risk developing severe meningitis, if infected. All common Real-Time PCR block cyclers are usable for the test. Each reactions contains an internal amplification control. Therefore, false negative results due to inhibition of the reaction can be excluded. The test included the UNG enzyme to prevent re-amplification of contaminating amplicons. The test can also be used for the confirmation of bacterial colonies. The Real-Time PCR Test allows the detection of the species with high accuracy and is more precise than, cultural methods often are.

Supplier: Q BIOANALYTIC

Pseudomonas aeruginosa is tested usually with cultural methods. However, this may take several days to deliver results. Using this Real-Time PCR test you may have results already after a few hours using a DNA purification directly from filtered water without pre-enrichment. You can also use this test in combination with an appropriate pre- enrichment according to DIN EN ISO 20837. The kit is also suitable to confirm bacterial colonies on agar plates.

Supplier: Q BIOANALYTIC

Hazel-nut is allergenic for a considerable number of individuals. Using DNA based tests also processed food samples might be tested. Our Real-Time PCR test exhibits the required limit of detection laid down in the codex alimentarius. The Real-Time PCR kit has a limit of detection of ≤ 20 DNA-copies. The limit of detection can be influenced by sample matrix, level of processing, DNA-preparation and DNA-content.

Supplier: Q BIOANALYTIC

Sus scrofa (pork) halal and kosher food does exclude pork meet. To make sure that pork meat is really absent in complex food it is advisable to test for the presence of it using our Real-Time test. Positive DNA and all required components are included in the kit.

Supplier: Q BIOANALYTIC

Real-Time kit for the detection of Staphylococcus aureus.