Hai cercato: Reagenti per microscopia e colorazione

Supplier: Merck

Neo-Clear® rappresenta l'alternativa a basso rischio allo xilene. Praticamente inodore, compatibile con la maggior parte dei solventi ad elevata purezza e caratterizzato da un tasso di evaporazione inferiore rispetto allo xilene, quindi meno pericoloso per la salute.

Supplier: Merck

Cryoembedding media for microscopy M-Freeze™ is used in the routine for preparing specimens of unfixed tissue (native material) of human origin for frozen-section analysis. It is also used for preparing cryo sections for enzyme and immunohistology methods to avoid fixation artefacts.

Supplier: SIGMA ALDRICH MICROSCOPY

Immersion oil has been used in epifluorescent microscopy and cell identification.

Supplier: SIGMA ALDRICH MICROSCOPY

Spurr resin is a hydrophobic resin. It is less viscous than epon mixtures and is easier to dispense. The low viscosity of the resin contributes to its penetration in tissue, mineral and dense structures. A low viscosity of 60 cps also allows easy penetration into a variety of difficult materials.

Supplier: SIGMA ALDRICH MICROSCOPY

HistoChoice® is a patented, complex mixture of agents that allow tissues to retain their structure, antigenic sites and nucleic acid sites. This formulation in non-citrus, contains no mercury, formaldehyde or glutaraldehyde.

Supplier: SIGMA ALDRICH MICROSCOPY

Acid Alcohol. Acidified alcohol solution for the differentiation of regressive hematoxylin stains.

Supplier: VWR Chemicals

When sectioning frozen tissue for rapid surgical biopsy diagnosis or for research (typically in Histochemistry), the tissue sample must be firmly attached to the microtome stage or chuck. Embedding material such as OCT are preferred as these also provide a supportive and protective aid to sectioning.

Supplier: VWR Chemicals

mezzo montante ad elevata viscosità per criotomia.

Supplier: Avantor

Mezzo di montaggio per la copertura dei vetrini.

Supplier: Merck

DPX new is a synthetic non aqueous mounting medium for microscopy. In its formulation, the teratogenic plasticiizer dibutyl phthalate (DBP) has been avoided, which renders the composition safer for the environment and more user friendly than the conventionally used mounting agents such as DPX.

Supplier: SIGMA ALDRICH MICROSCOPY

Fluoroshield™ with DAPI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.

Supplier: SIGMA ALDRICH MICROSCOPY

Fluoromount™ Aqueous Mounting Medium for use with fluorescent dye-stained tissue.

Supplier: SIGMA ALDRICH MICROSCOPY

Bouin's solution is an excellent fixative for preserving soft and delicate structures. It is prepared with saturated picric acid, formaldehyde and acetic acid.

Supplier: SIGMA ALDRICH MICROSCOPY

This is a mounting medium (contains 45% acrylic resin and 55% xylenes) used in microscopy. It is an adhesive and specimen preservative that can be used manually and in automated coverslipping equipment. Special purpose adhesive and sealant for use with glass, non plastic and other non-reactive materials.

Supplier: SIGMA ALDRICH MICROSCOPY

D.P.X mounting medium is a mixture of distyrene (polystyrene), a plasticizer (tricresyl phosphate), and xylene. It facilitates the protection of stains and can dry quickly.

Supplier: SIGMA ALDRICH MICROSCOPY

DPX Mountant for histology has been used as a slide mountant to fix sections.

Supplier: SIGMA ALDRICH MICROSCOPY

Differentiation solution comprises acidified ethanol or isopropanol that is used to decolourise hematoxylin stain. The duration of exposure to the solution determines the degree of decolourisation. Differentiation is a crucial step in hematoxylin-eosin staining to achieve the proper colour contrast necessary for visualisation, by selectively removing excess hematoxylin from the tissue section and leaving behind the desired staining intensity.

Supplier: Merck

Used to increase lens effectiveness

Supplier: Merck

La durezza definita e l'uniformità del materiale da tagliare sono requisiti cruciali per la qualità del taglio. Tali esigenze possono essere soddisfatte mediante una serie di sostanze idonee, quali paraffine, resine, gelatina e celloidina.

Supplier: SIGMA ALDRICH MICROSCOPY

Fluoroshield™ with 1,4-Diazabicyclo[2.2.2]octane is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.

Supplier: SIGMA ALDRICH MICROSCOPY

Immersion oil is used for high resolution (1000X) light microscopy work under oil immersion objective lens.

Supplier: SIGMA ALDRICH MICROSCOPY

ImmunoHistoMount™ is a permanent aqueous mounting medium designed for tissue sections and cell smears with peroxidase and alkaline phosphatase chromogens that cannot be dehydrated with organic solvents.

Supplier: SIGMA ALDRICH MICROSCOPY

PolyFreeze is a water-soluble support matrix and a form of embedding medium for frozen sectioning. PolyFreeze sections flawlessly at routine frozen specimen thickness of 5-6 μ and leaves no slide residue. It provides for -20 ºC cryostat sectioning in rapid set embedment. This medium freezes quickly supporting the tissue for sectioning at 3 mm and up with no cracking of the matrix at temperatures from -8 °C to -25 °C.

Supplier: Merck

Le soluzioni di montaggio non acquose, ad esempio Neo-Mount®, sono destinate al montaggio permanente e alla conservazione a lungo termine dei campioni. I vetrini processati nei laboratori patologici devono essere conservati fino a 10 anni, di conseguenza i risultati della colorazione devono essere stabili e non possono ammettere scolorimenti. La soluzione di montaggio anidra Neo-Mount® per microscopia è estremamente stabile e prodotta con solventi a base di miscele di idrocarburi alifatici. Poiché contiene un componente privo di aromatici in sostituzione dello xilene, la soluzione Neo-Mount® può essere combinata esclusivamente con Neo-Clear®. Non utilizzare lo xilene in fase di montaggio, in quanto causa l'offuscamento e la striatura dei vetrini. In aggiunta, posizionando i vetrini disidratati sulla carta filtrante per circa 1 minuto prima del montaggio, si impedisce l'eventuale eccesso di Neo-Clear®, in quanto sotto il vetrino coprioggetto potrebbero formarsi bolle di aria. Tale azione preventiva è da eseguire anche quando si montano i vetrini con macchine montavetrini; in quest'area, un'efficace eliminazione di NEO-CLEAR® si ottiene incubando i vetrini per un minuto in un rack per vetrini vuoto.

Supplier: Merck

Entellan® new è una nuova soluzione di montaggio per montavetrini ed è particolarmente adatta per strumenti commerciali di montaggio automatizzati utilizzati unitamente a montavetrini. Ha un range di viscosità di 250 - 600 mPas, il cod. prod. 1,00869 ha un range inferiore. Questo basso range di viscosità migliora e accelera l'applicazione, in quanto non occorre ripristinare il tasso di erogazione o il volume dopo il primo ciclo con un nuovo flacone di Entellan® new.

Supplier: Merck

Aquatex is used for e.g. LEUCOGNOST stained specimens. For preservation purposes and subsequent optimal microscopy, the stained sections are normally mounted using a suitable mounting agent and covered with a cover glass.

Supplier: SIGMA ALDRICH MICROSCOPY

The Epoxy Embedding Medium Kit is a very widely used embedding medium for electron microscopy. The embedding formulation originally published by Luft (1961) is excellent for both plant and animal tissues. Due to the low viscosity of the resin, it penetrates the tissue specimen faster than Araldite and other polymers. It hardens easily and uniformly at low temperatures with the addition of dodecenylsuccinic anhydride (DDSA), methyl nadic anhydride (MNA), and the accelerator 2,4,6-tris(dimethylaminomethyl)phenol (DMP-30). Slight shrinkage may occur during curing. This kit is useful for embedding a variety of tissues as a wide range of hardness can be obtained with this resin to suit a specific tissue type by using two different anhydride curing agents (DDSA and MNA).

Supplier: SIGMA ALDRICH MICROSCOPY

For use with Osteo-bed bone embedding kit. Solvent for removal of plastic from sections prior to rehydration and staining. Most histochemical and immunohistochemistry staining protocols require the removal of methyl methacrylate (MMA) and rehydration of mounted sections for improved stain penetration and greater contrast.

Supplier: SIGMA ALDRICH MICROSCOPY

CC/mount is an aqueous based permanent mounting medium with a very high refractive index, which when applied to the stained tissue sections can store the tissue specimens permanently without the fading of the chromogens. Because of the superior refractive index of CC/mount, tissues mounted in this medium look like specimens mounted with organic based mounting media. No coverslipping is required with CC/mount. However, if coverslipping is required, dried CC/mount can be post mounted by using an organic based mounting medium. CC/mount permits the long-term storage of antigens localised using organic insoluble chromogen substrates, including AEC, DAB, Fast Red, BCIP/NBT, BCIP/INT and fluorescent dyes like FITC and phycobiliproteins. The high pH of CC/mount ensures increased stability of fluorescence intensity.

Supplier: VWR Chemicals

High viscosity mounting media for cryotomy. Less viscous than O.C.T.