You searched for: Reagenti per elettroforesi

Supplier: Thermo Fisher Scientific

PageRuler™ pre-stained protein ladders are a mixture of ten blue-, orange- and green-stained proteins (10 to 180 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.

Supplier: AAT Bioquest

The Gelite™ 100 bp to 1 kb DNA ladder is designed to help size and estimate the mass of double-stranded DNA and PCR products within the 100 to 10000 base pair range on agarose or polyacrylamide gels.

Supplier: AAT Bioquest

ReadiUse™ 1 kb Plus DNA ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading.

Supplier: EDVOTEK

20 µg for 20 gels.

Supplier: LONZA

Lonza SimplyLoad™ DNA ladders are ready to load directly onto the gel to maximise time and convenience. Each band has an ideal 50% G-C content for rapid, precise sizing and sharp resolution. Free from contaminating plasmid DNA so they have minimal background.

Supplier: G-Biosciences

The Apoptotic DNA Ladder Kit contains the reagents necessary for the preparation of cellular fractions for the production of apoptosis nucleosomal DNA ladders. The kit does not require toxic phenol and DNA ladder is prepared either with or without genomic DNA.

Supplier: Thermo Fisher Scientific

Ethidium bromide destaining bags are used to safely remove ethidium bromide from nucleotide solutions and gels during overnight treatment. Each bag extracts up to 5 mg of Ethidium Bromide from solution, thus minimising the exposure of research personnel to this material.

Supplier: G-Biosciences

After Coomassie Brilliant Blue staining process, the band intensity may be further enhanced by de-staining the stained gel in our CBB De-Staining Solution.

Supplier: Cytiva

Amersham™ ECL™ Prime is a highly sensitive Western blotting detection reagent characterised by stable signal emission, allowing for the possibility of repeated exposures and making it easier to process several blots in the same experimental run. Amersham™ ECL™ Prime delivers a detection system that is sensitive, stable, precisely quantitative across a wide dynamic range of protein levels, and conservative in its consumption of expensive antibody reagents. The high performance of Amersham™ ECL™ Prime is due to the presence of an enhancer in the reagent, increasing enzyme turnover, and thereby significantly increasing both signal intensity and duration. The light signal is further intensified by the addition of a catalyst.

Supplier: Cytiva

Pharmalyte® carrier ampholytes, prepared by the co-polymerization of glycine, glycylglycine, amines and epichlorohydrin, are available in five broad-range and four narrow-range pH intervals.

Supplier: Cytiva

Amersham ECL Blocking Agent is for blocking nitrocellulose and PVDF membranes to be used with Amersham ECL, Amersham ECL Prime and Amersham ECL Select Western Blotting Detection Reagents

Supplier: MP Biomedicals

This antibody diluent is compatible with common stabilising compounds at pH 7,3.

Supplier: Cytiva

Amersham ECL detection reagent uses enhanced luminol-based detection suitable for all routine confirmatory Western blotting experiments.

Supplier: Cytiva

Each kit contains the detection reagent, HRP conjugated antibodies, and blocking agent sufficient for detection of 1000 cm² membrane.

Supplier: Cytiva

CyDye™ Mono-Reactive Maleimide Fluorescent Dyes are intensely fluorescent and highly water soluble, providing significant advantages over other existing fluorophores.

Supplier: Cytiva

Amersham ECL Select is a high sensitivity reagent for chemiluminescent Western blotting detection.

Supplier: Cytiva

CypHer5E is a red-excitable, pH-sensitive cyanine dye derivative that is minimally fluorescent at a basic pH and maximally fluorescent at an acidic pH.

Supplier: Cytiva

Enhanced chemiluminescence (ECL) detection reagents for reliable detection of medium and high expression proteins.

Supplier: Cytiva

Cy™ fluorescent amidites for incorporation of Cy3, Cy3,5, Cy5, Cy5,5 fluorescent dyes at any position in an oligonucleotide during synthesis.

Supplier: Cytiva

Chemiluminescence detection reagent for detection of high to medium abundant proteins.

Supplier: Cytiva

Amersham ECL Prime Blocking Reagent is blocking Amersham Protran NC and Amersham Hybond PVDF membranes used with Amersham ECL start, Amersham ECL, Amersham, ECL Prime, Amersham ECL Select and Amersham ECL Plex.

Supplier: Cytiva

ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 minute chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.

Supplier: Cytiva

ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 minute chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.

Supplier: Cytiva

Il kit combinato per Western blotting Amersham ECL Plex™ offre una soluzione completa per Western blotting fluorescente, con formulazione migliorata dei coniugati Cy3 per ridurre il rumore di fondo.

Supplier: Cytiva

The appearance of streaks that distort 2-D electrophoresis maps is a common problem, occurring most frequently when running gels that contain regions greater than pH 7,0. Increased sample load, increased length of the IPG strip, or using a narrower pH gradient worsens the problem. Extra spots on 2-D gels, caused by non specific oxidation of proteins, is another difficulty encountered when running gels containing basic regions. Both streaking and non specific oxidation result in poorly resolved protein patterns and reduced reproducibility between electrophoresis runs.

Supplier: Cytiva

ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 min chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.

Supplier: Cytiva

AlkPhos Direct™ Labelling and detection systems are based on the rapid, direct labelling of DNA or RNA probes with thermostable alkaline phosphatase. AlkPhos Direct™ combines the convenience of direct enzyme labelling (no blocking or antibody stages) with those of alkaline phosphatase detection (long light output and high sensitivity).

Supplier: Biotium

A visible three- colour protein marker for SDS-PAGE or western blotting, with 12 bands ranging from 8 to 245 kDa.

Supplier: Thermo Fisher Scientific

This wide range molecular weight marker contains 60 ng/μl of 21 fragments from 100 bp to 12000 bp. The bands appear in 100 bp increments from 100 to 1000 bp and in 1000 bp increments from 1 to 12 kb. Bands at 1 kb and 5 kb stain brighter to aid identification.

Supplier: G-Biosciences

DNAmark™ 100 bp Plus ladder consists of 14 DNA fragments ranging in size from 100 to 3000 base pairs (bp). The 500 bp and 1200 bp fragment are present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 µl loading, all fragments except 500 bp and 1200 bp are 40 ng. The 500 bp and 1200 bp fragment are 100 ng.