Supplier: Enzo Life Sciences

AMPIGENE® DNA ladder contains DNA fragments ranging from 100 bp to 1500 bp that can be used as molecular weight standards for agarose gel electrophoresis. The bands are evenly spaced and are intensified at 500 bp and 1500 bpto serve as easily identifiable reference bands. The approximate mass of DNA in each of the bands is provided, assuming 5 µl load, for determining the approximate mass of DNA in samples with comparable intensity and sizes.

Supplier: Enzo Life Sciences

AMPIGENE® DNA ladder contains DNA fragments ranging from 100 bp to 10000 bp that can be used as molecular weight standards for agarose gel electrophoresis. The bands are evenly spaced and are intensified at 500 bp, 1500 bp and 3000 bp to serve as easily identifiable reference bands. The approximate mass of DNA in each of the bands is provided, assuming 5 µl load, for determining the approximate mass of DNA in samples with comparable intensity and sizes.

Supplier: Thermo Fisher Scientific

Thermo Scientific™ GeneRuler™ and O'GeneRuler™ DNA ladders are mixtures of chromatography-purified individual DNA fragments. The GeneRuler™ line includes the most popular ladders, such as the 100 bp and 1 kb DNA Ladder. GeneRuler™ DNA ladders are available in two formats: Conventional (provided in TE buffer) and a ready-to-use format (premixed with 6X DNA Loading Dye which contains bromophenol blue and xylene cyanol FF). O'GeneRuler™ DNA ladders are another ready-to-use version of GeneRuler™ DNA ladders that are premixed with 6X Orange DNA Loading Dye.

Supplier: MP Biomedicals

The EtBr Greenbag™ Disposal Kit allows rapid and trouble-free concentration of ethdium bromide from large volumes of solutions into a small 'teabag' containing activated carbon. The Greenbag™ is then conveniently disposed of along with other solid hazardous wastes.

Supplier: G-Biosciences

G-Biosciences' Destain I contains approximately 45% methanol and 9% glacial acetic acid. G-Biosciences' Destain II contains approximately 5% methanol and 7% acetic acid.

Supplier: Thermo Fisher Scientific

The Pierce™ Fast Western Blot Kit, SuperSignal™ West Femto Substrate contains optimised reagents that shorten the time to perform a typical Western blot from 4 hours to ~60 minutes.

Supplier: Thermo Fisher Scientific

Saponin permeating solution, is used as a cell permeabilising agent.

Supplier: G-Biosciences

Perfect-FOCUS™ is based on quantitative precipitation and concentration of protein solutions using UPPA™ (Universal Protein Precipitation Agent) and has been specifically developed for preparing lower conductivity protein samples for iso-electric focusing gels. Perfect-FOCUS™ is designed to clean and remove interfering agents from protein samples and concentrate protein solutions that give poor protein spot resolution during 2D electrophoresis.

Supplier: Merck Millipore (Oncogene)

This Western-Re-Probe™ reagent is validated for use in immunoblotting.

Supplier: Thermo Fisher Scientific

QuantaRed™ Enhanced Chemifluorescent HRP Substrate contains chemical enhancers to increase the fluorescent yield and sensitivity of 10-acetyl-3,7-dihydroxyphenoxazine (ADHP) chemifluorescence.

Supplier: Thermo Fisher Scientific

SSC solution is commonly used in nucleic acid hybridization techniques at concentrations ranging from 0,1X to 20X depending on the application. It is used to facilitate transfer of nucleic acids to membranes in Southern and Northern blotting, dot blotting, and colony and plaque lifts.

Supplier: Rockland Immunochemicals

FemtoMax™ is an extremely sensitive, non radioactive, and enhanced luminol-based chemiluminescent substrate for the detection of horseradish peroxidase (HRP). It allows for the detection of femtogram (10 to 15) amounts of antigen using photographic film or other imaging methods including highly sensitive CCD cameras.

Supplier: Thermo Fisher Scientific

The Fast Western Blot Kit, SuperSignal™ West Pico Substrate contains optimised reagents and protocol for picogram-level detection and and Western blot development less than 1 hour after gel transfer.

Supplier: Rockland Immunochemicals

TrueBlot® Western blot kits contain the critical supporting reagents, buffers, and substrates for immunoprecipitation and Western blotting of samples using TrueBlot® second step immunoblotting reagents in conjunction with the user's own primary IP antibody and primary (mouse, goat or rabbit IgG) Western blotting antibody.

Supplier: Thermo Fisher Scientific

The Fast Western Blot Kits, SuperSignal™ West Dura provide reagents and protocol for reliable and rapid, 1 hour chemiluminescent Western blotting at femtogram levels of sensitivity. These complete Fast Western Blot Kits requires minimal hands on time and yield results comparable to classical Western blotting with SuperSignal™ West Dura Chemiluminescent Substrate. This substrate produces a signal that is detectable using photographic or other imaging methods. The developed blots can be repeatedly exposed and stripped of the primary antibody and reprobed. The complete kit contains all the reagents necessary to block and probe a Western blot with mouse or rabbit primary antibody.

Supplier: PanReac AppliChem

The CheLuminate-HRP PicoDetect is a complete kit with ready to use reagents for chemiluminescent detection of immobilised proteins (Western blot) or immobilised nucleic acids (Southern or Northern blot), conjugated with horseradish peroxidase (HRP) directly or indirectly.

Supplier: Rockland Immunochemicals

These Chemiluminescent Western Blot Kits allow for the detection of primary polyclonal or monoclonal antibodies provided by the user. After protein separation and transfer, the membrane is probed with primary antibody. Detection of the membrane-bound primary antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialised formulation of luminol, an extremely sensitive, non radioactive substrate that emits light and allows visualisation using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems. Because of the extremely high sensitivity of the kit, primary and secondary antibodies can be used at greater dilutions. Primary antibodies currently diluted 1:1000 using an ECL™ substrate is the equivalent of a 1:5000 dilution using the FemtoMax™ substrate.

Supplier: G-Biosciences

Kingdom blot is prepared using total protein extracted from a variety of species, where whole species is not available the whole liver lysate is used. These blots can be used to identify similar proteins in other species, including human, chicken, frog, worm, Drosophila, yeast, Aspergillus, E. coli, Rhizobium and green algae.

Supplier: Thermo Fisher Scientific

For chemiluminecent HRP-based detection of western blotting systems. It is soluble in most polar organic solvents but is insoluble in water.

Supplier: Merck Millipore (Novagen)

The Perfect Protein™ HRP Western Blot Kit includes an HRP or AP conjugate in addition to Perfect Protein™ Western markers.

Supplier: G-Biosciences

Many lysis buffers and reagents used in sample preparation are incompatible with routinely used electrophoretic analysis. The presence of contaminants, or interfering agents, such as salts, acids, bases and detergents, result in band distortion and poor protein resolution. As a result, SDS-PAGE gels are hard to analyse and lack reproducibility. PAGE-Perfect™ is a simple, two-step method for concentrating, cleaning and preparing protein solutions for running publication quality gels.

Supplier: Thermo Fisher Scientific

The Spectra™ Multicolour Broad Range Protein Ladder is a 4-colour protein standard containing 10 prestained proteins (10 to 260 kDa) for use in gel electrophoresis and Western blotting.

Supplier: EDVOTEK

For 20 gels.

Supplier: AAT Bioquest

The Gelite™ 50 bp DNA Ladder is ideal for sizing and approximating the mass of double-stranded DNA within the 50 to 1500 bp range on agarose or polyacrylamide gels.

Supplier: AAT Bioquest

ReadiUse™ 100 bp DNA ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading.

Supplier: Biotium

DNA ladders in TE buffer are used for sizing linear double-stranded DNA fragments separated by gel electrophoresis. The 1 kb DNA ladder is suitable for sizing DNA fragments from 250 bp to 10 kb, while the 100 bp DNA ladder is suitable for sizing DNA fragments from 100 bp to 1500 bp.

Supplier: AAT Bioquest

The ProLite™ 5 to 245 kD protein ladder is a high-quality three-colour protein standard consisting of 13 prestained proteins.

Supplier: Thermo Fisher Scientific

PageRuler™ Plus pre-stained protein ladders are a mixture of nine blue-, orange- and green-stained proteins (10 to 250 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.

Supplier: Thermo Fisher Scientific

PageRuler™ pre-stained protein ladders are a mixture of ten blue-, orange- and green-stained proteins (10 to 180 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.

Supplier: AAT Bioquest

The Gelite™ 100 bp to 1 kb DNA ladder is designed to help size and estimate the mass of double-stranded DNA and PCR products within the 100 to 10000 base pair range on agarose or polyacrylamide gels.