Reagenti per elettroforesi
L’elettroforesi su gel è un metodo utilizzato in biologia molecolare per separare DNA e proteine mediante una corrente elettrica applicata in base al peso molecolare o alla dimensione dei frammenti. I vari reagenti, tra cui i marcatori di peso molecolare, i reagenti per blotting e i reagenti per la rimozione delle macchie, migliorano la precisione di rilevamento dei risultati dell’elettroforesi grazie a una visualizzazione potenziata. I reagenti per elettroforesi offerti sono ideali per l’uso nei campi della biologia molecolare, dell’immunogenetica e di altre discipline della biologia molecolare.
Peqlab peqGOLD, Protein Marker II (Unstained)
Supplier: VWR Chemicals
Protein Marker II for accurate molecular weight determination of proteins by SDS-PAGE.
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VWR® Soluzione di fissaggio per focalizzazione isoelettrica in gel di poliacrilammide, Reag. Ph. Eur. 1138700
Supplier: VWR Chemicals
VWR®, Soluzione di fissaggio per focalizzazione isoelettrica in gel di poliacrilammide
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Sacchetti per decolorazione
Supplier: VWR Chemicals
I sacchetti per decolorazione per rimuovere in modo sicuro il bromuro di etidio dalle soluzioni durante trattamenti notturni. Ciascun sacchetto consente di estrarre fino a 5 mg di bromuro di etidio dalla soluzione, riducendo quindi al minimo l'esposizione del personale ai materiali tossici. La speciale miscela assorbente trattiene le molecole asciutte in un sacchetto semplice da rimuovere e incenerire.
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Peqlab peqGOLD, Protein Marker V (Pre-Stained)
Supplier: VWR Chemicals
Protein Marker V (pre-stained) for monitoring protein separation, transfer efficiency and size determination.
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VWR® EZ-Vision®, DNA Ladder, 1 kb
Supplier: VWR Chemicals
EZ-Vision® 1 kb DNA ladder is a pre-mixture of DNA marker, loading buffer and a proprietary fluorescent DNA visualisation dye that can be applied directly to the gel. This non toxic, non mutagenic dye eliminates the need for ethidium bromide staining. The 1 kb ladder consists of 10 double-stranded bands with molecular weights between 200 and 10 000 bp. The intensity of individual bands varies to facilitate easy identification.
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Peqlab peqGOLD, Protein Marker I (Unstained)
Supplier: VWR Chemicals
peqGOLD Protein Marker I is a mixture of seven purified proteins which are re-dissolved 'ready to use' in loading buffer. The proteins resolve into sharp, tight bands in the range of 14,4 to 116,0 kDa when analysed by SDS-PAGE and stained with Coomassie Brilliant Blue R-250.
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VWR®, DNA ladders
Supplier: VWR Chemicals
VWR® DNA ladders are convenient dsDNA ladders supplied in a loading buffer, which are ready to use on agarose and SDS DNA gels.
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VWR®, Protein marker VI (pre-stained), peqGOLD
Supplier: VWR Chemicals
peqGOLD pre-stained Protein Marker VI is a mixture of 10 coloured proteins. These proteins are recombinantly produced in E.coli, highly purified and supplied 'ready to use' in loading buffer. The protein concentrations are optimised to yield well-defined bands directly visible in SDS polyacrylamide gels.
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VWR® Ready Ladder™ DNA Ladder, 50 bp
Supplier: VWR Chemicals
Marcatore con 10 frammenti da 50 a 500 bp per una semplice identificazione della banda. Formula pronta all'uso premiscelata con colorante di caricamento.
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Peqlab peqGOLD, DNA Ladder, 100 bp Plus
Supplier: VWR Chemicals
peqGOLD DNA ladder 100 bp plus is ideal for size determination in the range of 100 to 3000 base pairs. There are no unspecific bands besides the fragments described. 1000 and 500 bp bands have a higher DNA content and serve as a size reference.
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Peqlab peqGOLD, Protein Marker IV (Pre-Stained)
Supplier: VWR Chemicals
peqGOLD pre-stained protein marker IV is a mixture of 10 recombinant, highly purified coloured proteins supplied 'ready to use' in loading buffer. The protein concentrations are optimised to yield well-defined bands directly visible in SDS-polyacrylamide gels.
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VWR® RiboReady™, Color RNA Ladder, 1 kb
Supplier: VWR Chemicals
RiboReady™ Color 1 kb RNA ladder consente una dimensionatura precisa delle molecole di RNA in gel di acrilammide denaturanti.
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VWR®, DNA ladder
Supplier: VWR Chemicals
VWR® I DNA ladder sono forniti in tampone di caricamento pronto all’uso su gel di DNA in agarosio e poliacrilammide. I ladder sono compatibili con sistemi di elettroforesi TBE, TAE, SB e LB.
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VWR® PCR DNA Marker
Supplier: VWR Chemicals
PCR DNA Marker™ con 8 frammenti da 50 a 2000 bp. Il marcatore contiene bande di riferimento ad alta intensità ideali per determinare rapidamente le dimensioni dei prodotti di PCR e per digestione con enzimi di restrizione. È sufficiente miscelare 10 μl di PCR DNA Marker™ con la quantità richiesta di tampone di caricamento e caricare nel pozzetto. Materiale sufficiente per 100 saggi.
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Chemicon® ReBlot Plus Kits
Supplier: Merck
Western blotting is a commonly used technique for studying protein function and localisation. Typically, protein samples are electrophoresed on SDS-page and transferred to a membrane such as nitrocellulose or nylon, where they are probed with specific antibodies. Unlike nucleic acid based technologies, which allow reuse of southern and northern blots, it has been difficult to reuse western blots.
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DNA ladder 50 to 1500 bp, AMPIGENE®
Supplier: Enzo Life Sciences
AMPIGENE® DNA ladder contains DNA fragments ranging from 50 bp to 1500 bp that can be used as molecular weight standards for agarose gel electrophoresis. The bands are evenly spaced and are intensified at 200 bp, 500 bp and 1500 bpto serve as easily identifiable reference bands. The approximate mass of DNA in each of the bands is provided, assuming 5 µl load, for determining the approximate mass of DNA in samples with comparable intensity and sizes.
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