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Reagenti per elettroforesi

Gel electrophoresis is a method used in molecular biology to separate DNA and proteins through an electrical current applied based on molecular weight or fragment size. The various reagents, including molecular weight markers, blotting reagents, and stain removal reagents, improve the detection accuracy of electrophoresis results through enhanced visualization. The electrophoresis reagents offered are ideal for use in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.

VWR® RiboReady™, Color RNA Ladder, 1 kb

VWR® RiboReady™, Color RNA Ladder, 1 kb

Supplier: VWR Chemicals

RiboReady™ Color 1 kb RNA ladder allows precise sizing of RNA molecules in denaturing acrylamide gels.

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Restore™ Western Blot Stripping Buffer

Restore™ Western Blot Stripping Buffer

Supplier: Thermo Fisher Scientific

Although optimising assay conditions is the best way to achieve optimum results, re-performing the gel electrophoresis process to test each new primary antibody or antibody concentration is time-consuming and expensive. Restore™ Western blot stripping buffer offers an effective alternative.

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Western blotting detection reagents, ECL western blotting substrate

Western blotting detection reagents, ECL western blotting substrate

Supplier: Thermo Fisher Scientific

ECL™ Western blotting substrate is an entry-level Western blotting substrate.

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Pierce™ Chemiluminescent Nucleic Acid Detection Module Kit

Pierce™ Chemiluminescent Nucleic Acid Detection Module Kit

Supplier: Thermo Fisher Scientific

The Pierce™ Chemiluminescent nucleic acid detection module is a complete system for the detection of biotin-labeled nucleic acids for various blotting applications, including Northern/Southern blots, ribonuclease protection assays (RPAs), and electrophoretic mobility shift assays (EMSAs) or gel-shift assays.

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West HisProbe kit, SuperSignal™

West HisProbe kit, SuperSignal™

Supplier: Thermo Fisher Scientific

SuperSignal™ West HisProbe Kit contains HisProbe-HRP conjugate and reagents required for detection of polyhistidine-tagged protein by Western blotting. HisProbe-HRP Conjugate is a nickel (Ni²⁺)-activated derivative of horseradish peroxidase (HRP) that enables direct, IMAC-based detection of His-tagged proteins.

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DAB substrate kit, metal enhanced, Pierce™

DAB substrate kit, metal enhanced, Pierce™

Supplier: Thermo Fisher Scientific

The Pierce™ Metal enhanced DAB substrate kit uses cobalt and nickel chloride in a special formulation of diaminobenzidine peroxidase substrate that yields intense colour for immunohistochemical staining.

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Peqlab peqGOLD, DNA Ladder, 1 kb

Peqlab peqGOLD, DNA Ladder, 1 kb

Supplier: VWR Chemicals

DNA ladder for quantitative and qualitative analysis of dsDNA.

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VWR® EZ-Vision®, DNA Ladder, 100 bp

VWR® EZ-Vision®, DNA Ladder, 100 bp

Supplier: VWR Chemicals

EZ-Vision® 100 bp DNA ladder is a pre-stained molecular weight ladder in loading buffer that allows immediate visualisation of DNA ladder post-electrophoresis.

    
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VWR®, DNA ladder, 100 bp

VWR®, DNA ladder, 100 bp

Supplier: VWR Chemicals

Ladder con 13 frammenti da 100 a 3000 bp per una semplice identificazione della banda.

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Peqlab peqGOLD, DNA Ladders, 50 bp

Peqlab peqGOLD, DNA Ladders, 50 bp

Supplier: VWR Chemicals

DNA ladder for quantitative and qualitative analysis of dsDNA.

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Peqlab peqGOLD, DNA Ladder, 100 bp

Peqlab peqGOLD, DNA Ladder, 100 bp

Supplier: VWR Chemicals

DNA ladder for quantitative and qualitative analysis of dsDNA.

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VWR®, Protein marker III (pre-stained), peqGOLD

VWR®, Protein marker III (pre-stained), peqGOLD

Supplier: VWR Chemicals

Protein Marker III for monitoring protein separation, transfer efficiency and size determination.

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VWR® DNA Molecular Weight Marker, High-Range

VWR® DNA Molecular Weight Marker, High-Range

Supplier: VWR Chemicals

Marcatore MW (peso molecolare) per DNA, gamma alta: 10 frammenti da 1.503 a 48.502 bp. Materiale sufficiente per 75 – 150 saggi.

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Peqlab peqGOLD, Protein Marker II (Unstained)

Peqlab peqGOLD, Protein Marker II (Unstained)

Supplier: VWR Chemicals

Protein Marker II for accurate molecular weight determination of proteins by SDS-PAGE.

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VWR® RiboReady™ Color Micro RNA Ladder

VWR® RiboReady™ Color Micro RNA Ladder

Supplier: VWR Chemicals

RiboReady™ Color Micro RNA ladder allows precise sizing of RNA molecules in denaturing acrylamide gels. The apparent sizes of bands in the RiboReady™ Color Micro RNA ladder are 100, 75, 50, 40, 30 and 20 bases in length.

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Peqlab peqGOLD, DNA Ladder Mix

Peqlab peqGOLD, DNA Ladder Mix

Supplier: VWR Chemicals

DNA ladder for quantitative and qualitative analysis of dsDNA.

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VWR®, DNA marker, 1 kb

VWR®, DNA marker, 1 kb

Supplier: VWR Chemicals

DNA marker consists of 11 fragments ranging from 500 to 10000 bp for easy band identification.

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DNA ladder, O'RangeRuler™

DNA ladder, O'RangeRuler™

Supplier: Thermo Fisher Scientific

O'RangeRuler™ DNA ladders are step ladders that consist of basic unit repeats of 10, 15, 20, 50, 100, 200, or 500 bp. The ladders have a higher density of bands at a specific DNA size range and are therefore suitable for more accurate sizing of DNA in agarose or non denaturing polyacrylamide gels.

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Lambda DNA/HindIII Marker

Lambda DNA/HindIII Marker

Supplier: Thermo Fisher Scientific

Thermo Scientific Lambda DNA/HindIII Marker is recommended for sizing of linear double-stranded large DNA fragments in agarose gels. Lambda DNA is digested to completion with the appropriate restriction enzyme(s) and purified and dissolved in storage buffer. DNA fragments contain various sticky ends depending on the restriction enzyme used for preparation of the marker.

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Lambda DNA phage, Hind III digest

Supplier: Rockland Immunochemicals

Lambda DNA fragments prepared by the digestion of purified lambda DNA with the restriction endonuclease Hind III.

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Protein ladders (pre-stained), ScanLater™

Protein ladders (pre-stained), ScanLater™

Supplier: Molecular Devices

The ScanLater™ Western blot protein ladder is a mixture of seven biotinylated recombinant proteins at 10, 20, 40, 60, 80, 100, and 140 kD.

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DNA ladders

Supplier: Biotium

Ready to use DNA ladders are supplied in 1X loading buffer at an optimal concentration for loading GelRed™ and GelGreen™ precast gels.

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Upstate® Autorad Orientation Markers for Western Blotting

Upstate® Autorad Orientation Markers for Western Blotting

Supplier: Merck

Autorad orientation markers for use on immunoblots in detection of Chemiluminescence in Western blotting, dot/slot blotting.

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DNA ladder, low range, MassRuler

DNA ladder, low range, MassRuler

Supplier: Thermo Fisher Scientific

MassRuler Low Range DNA Ladder, ready to use, 80 to 1031 bp, is specially designed for accurate quantification and sizing of DNA fragments by agarose gel electrophoresis.

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RNA ladder, high range, RiboRuler™

RNA ladder, high range, RiboRuler™

Supplier: Thermo Fisher Scientific

RiboRuler™ high range RNA ladder allows precise sizing of RNA molecules in denaturing agarose gels and Northern blotting.

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DNA ladders, ultra low range, FastRuler

DNA ladders, ultra low range, FastRuler

Supplier: Thermo Fisher Scientific

FastRuler ultra low range DNA ladder is specifically designed for fast sizing and approximate quantification of double-stranded DNA in 48 well (or 96-well) high throughput gels, as well as in conventional agarose gels. The ladder is a mixture of five blunt-ended chromatography-purified individual DNA fragments that are easily resolved in a short separation distance (10 to 20 mm) after an 8 to 14 minutes run on the appropriate agarose gel.

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Lambda DNA phage, E. coli W8850

Supplier: Rockland Immunochemicals

Lambda phage consists of a virus particle including a head (also known as a capsid), a tail and tail fibres. The head contains the phage's double-stranded circular DNA genome. The phage particle recognises and binds to its host E.coli causing DNA in the head of the phage to be ejected through the tail into the cytoplasm of the bacterial cell. Lambda phage DNA is commonly used as a DNA molecular weight marker. Digestion of the lambda phage genome with HindIII produces a set of 8 fragments consisting of 125, 560, 2000, 2300, 4300, 9400, and 23000 base pairs.

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DNA Ladder 100 to 10000 bp, AMPIGENE®

Supplier: ENZO LIFE SCIENCES

AMPIGENE® DNA ladder contains DNA fragments ranging from 100 bp to 10000 bp that can be used as molecular weight standards for agarose gel electrophoresis. The bands are evenly spaced and are intensified at 500 bp, 1500 bp and 3000 bp to serve as easily identifiable reference bands. The approximate mass of DNA in each of the bands is provided, assuming 5 µl load, for determining the approximate mass of DNA in samples with comparable intensity and sizes.

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DNA ladder 100 to 1500 bp, AMPIGENE®

Supplier: ENZO LIFE SCIENCES

AMPIGENE® DNA ladder contains DNA fragments ranging from 100 bp to 1500 bp that can be used as molecular weight standards for agarose gel electrophoresis. The bands are evenly spaced and are intensified at 500 bp and 1500 bpto serve as easily identifiable reference bands. The approximate mass of DNA in each of the bands is provided, assuming 5 µl load, for determining the approximate mass of DNA in samples with comparable intensity and sizes.

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DNA ladders, GeneRuler™ and O'GeneRuler™

DNA ladders, GeneRuler™ and O'GeneRuler™

Supplier: Thermo Fisher Scientific

Thermo Scientific™ GeneRuler™ and O'GeneRuler™ DNA ladders are mixtures of chromatography-purified individual DNA fragments. The GeneRuler™ line includes the most popular ladders, such as the 100 bp and 1 kb DNA Ladder. GeneRuler™ DNA ladders are available in two formats: Conventional (provided in TE buffer) and a ready-to-use format (premixed with 6X DNA Loading Dye which contains bromophenol blue and xylene cyanol FF). O'GeneRuler™ DNA ladders are another ready-to-use version of GeneRuler™ DNA ladders that are premixed with 6X Orange DNA Loading Dye.

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