Reagenti per estrazione di proteine

Una lisi cellulare efficiente e rese massime di estrazione proteica sono vitali per una purificazione di proteine ricombinanti di alta qualità. I cocktail di inibitori delle proteasi sono utilizzati per lisare cellule di mammifero, tamponi che solubilizzano le membrane plasmatiche e intracellulari, rompono i deboli legami intermolecolari e solubilizzano la maggior parte delle proteine. Questi reagenti di estrazione proteica, che rendono le procedure più facili da eseguire, possono anche essere utilizzati per isolare proteine dalle frazioni citoplasmatiche e nucleari di cellule coltivate.

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Endoproteinase Asp-N, excision grade, Pseudomonas fragi

Supplier: Merck Millipore (Calbiochem‎)

Native endoproteinase Asp-N from Pseudomonas fragi. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Metalloprotease that specifically hydrolyses proteins at the N-terminal side of aspartic acid and cysteic acid residues. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.

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Washing solution, Protein-OUT™

Supplier: G-Biosciences

Protein-OUT™ is a washing solution that cleans plastic and glassware used in the proteolytic digestion and peptide isolation for mass spectrometry analysis. The rinsing of plastic and glassware with Protein-OUT™ will remove interfering chemicals and peptides that can leach from the tube walls and interfere or suppress the mass spectrometry signal. In addition, rinsing with Protein-OUT™ removes inadvertent contamination with dust and keratin.

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Uniform latex particles for immobilisation of biomolecules, PL-Latex

Supplier: VARIAN

Uniform latex particles are used in a wide range of applications that exploit their physico-chemical properties in sophisticated immunoassay formats, both in medicine and veterinary science research.