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50 risultati per Reagenti per estrazione di proteine

Hai cercato: Reagenti per estrazione di proteine

Reagenti per estrazione di proteine

Protein purification reagents are for use in isolating one or more proteins from a complex mixture, usually cells, tissues, or whole organisms. This process is vital for function and structure characterization, as well as identifying interactions of the protein of interest. Important during the many steps, the protein purification reagents assist with cell lysis, separating the soluble protein components from cell debris, and finally separating the protein of interest from product- and process-related impurities.

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Piastre di precipitazione delle proteine J.T.Baker® BAKERBOND®

Piastre di precipitazione delle proteine J.T.Baker® BAKERBOND®

Supplier: Avantor

Le piastre di precipitazione delle proteine J.T.Baker® BAKERBOND® consentono una rapida rimozione delle proteine dai campioni biologici.

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Piastre per l'estrazione di liquidi supportati, J.T.Baker® BAKERBOND®

Piastre per l'estrazione di liquidi supportati, J.T.Baker® BAKERBOND®

Supplier: Avantor

Le piastre J.T.Baker® BAKERBOND® per l’estrazione di liquidi supportati (SLE) sono un'alternativa ideale e più veloce ai tradizionali metodi di estrazione liquido-liquido e permettono un eccellente recupero degli analiti dalle matrici biologiche.

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Concanavalin A agarose

Concanavalin A agarose

Supplier: G-Biosciences

Concanavalin A (Con A) agarose consists of Con A coupled to 6% agarose by the cyanogen bromide method. Con A is a tetrameric metalloprotein lectin isolated from Canavalia ensiformis (jack bean). It is used for the purification of glycoproteins, polysaccharides and glycolipids as it binds molecules containing α-D-mannopyranosyl, α-D-glucopyranosyl and sterically related residues. It has also be used in other application areas including purification of enzyme-antibody conjugates, purification of IgM and separation of membrane vesicles. As stated above, it is a metalloprotein and to maintain its binding characteristics the presence of both Mn²⁺ and Ca²⁺ is essential. Each subunit of Con A utilises one calcium and one manganese ion and these cations can be removed under acidic conditions abolishing the carbohydrate-binding activity.

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HRP Stability Conjugate Reagent

HRP Stability Conjugate Reagent

Supplier: Abcam

Abcam's HRP Stability Conjugate Reagent (ab171537) was developed specifically for Horseradish Peroxidase (HRP) conjugates in solution.

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Sample Diluent NBP

Sample Diluent NBP

Supplier: Abcam

Sample Diluent NBP is part of the reagents, controls and accessories range.

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TMB substrate 1X

TMB substrate 1X

Supplier: Abcam

TMB substrate recommended for use with the Matched Antibody Pair kits for sandwich ELISA.

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Streptavidin-HRP Solution

Streptavidin-HRP Solution

Supplier: Abcam

Streptavidin-HRP Solution (ab210901) is part of the reagents, controls and accessories range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.

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Pep-Extract™

Supplier: G-Biosciences

Following digestion of proteins with proteases, the addition of Pep-Extract™ rapidly elutes digested peptides in under 20 minutes. The eluted peptides can be used directly in mass spectrometry analysis without any additional clean up. Contains sufficient for 500 protein spots.

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Diluent M Concentrate (10x)

Diluent M Concentrate (10x)

Supplier: Abcam

Diluent M Concentrate (10X) (ab138908) is part of the reagents, controls & accessories range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.

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Immobilised D-galactose for lectin purification

Immobilised D-galactose for lectin purification

Supplier: G-Biosciences

Immobilised D-Galactose is designed for the rapid purification of lectins, galactosidases and other galactose-binding molecules. The resin is ideal for the purification of agglutinins, lectins, toxins, glactose-binding, N-acetylgalactosaminebinding or carbohydrate binding molecules. Specific applications include the purification of galactosidases, C‐type lectins, enterotoxins and cholera toxin.

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Proteomic protein controls

Supplier: G-Biosciences

Protein preparations specifically prepared for standardising electrophoresis methods and protocols. Substantially free from non-protein agents such as nucleic acids, detergents, salts, lipids and other common laboratory agents, the protein preparation has low conductivity (<50 μs). Supplied as dry protein pellets, simply re-hydrate and use. Proteomic protein sample controls are available for animal cells, E.coli, yeast, plant or as a control set that contains one 2 mg vial of each.

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Sample Diluent NBS

Sample Diluent NBS

Supplier: Abcam

Sample Diluent NBS (ab221827) is part of the reagents, controls and accessories range. High-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates are available.

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Jacalin for IgA purification

Jacalin for IgA purification

Supplier: G-Biosciences

Jacalin, or Artocarpus integrifolia lectin, is a tetrameric two-chain lectin with a molecular weight of 66 kDa. It is a α-D-galactose binding lectin purified from jack fruit (Artocarpus integrifolia) seeds.

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Protein stabilising cocktail, Pierce™

Protein stabilising cocktail, Pierce™

Supplier: Thermo Fisher Scientific

The Protein Stabilising Cocktail is a versatile stabilising solution that increases the shelf-life of purified or partially purified proteins during routine storage.

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PEGylated N-succinimidyl S-acetylthioacetate, SAT(PEG)4, Pierce™

PEGylated N-succinimidyl S-acetylthioacetate, SAT(PEG)4, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ SAT(PEG)₄ (PEGylated N-succinimidyl S-acetylthioacetate) is a sulphhydryl-addition reagent for covalent modification of primary amines.

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HeLa protein digest standard, Pierce™

HeLa protein digest standard, Pierce™

Supplier: Thermo Fisher Scientific

The HeLa Protein Digest Standard is a highly validated mammalian protein digest that may be used as a quality control sample for mass spectrometry (MS) analysis of complex proteomic samples.

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Pegylation reagents, Methyl-PEG-NHS-Maleimide, Pierce™

Pegylation reagents, Methyl-PEG-NHS-Maleimide, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ MM(PEG)n reagents are methyl-terminated PEG compounds activated with a maleimide group for covalent pegylation of sulfhydryls on proteins or assay surfaces.

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Recombinant protein A, peroxidase conjugated, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Recombinant Peroxidase Conjugated Protein A can be used to probe and detect rabbit and human antibodies, especially IgG isotypes, in Western blotting, ELISA, IHC and other immunoassay protocols.

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Recombinant protein A, Pierce™

Recombinant protein A, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Recombinant Protein A is useful as the basis for preparing various kinds of probes or affinity media for detection or purification of rabbit and human antibodies, especially IgG isotypes, in immunoassays and antibody purification protocols.

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Recombinant protein L, peroxidase conjugated, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Recombinant Peroxidase Conjugated Protein L can be used to probe and detect rabbit and human antibodies, especially IgG isotypes, in Western blotting, ELISA, IHC and other immunoassay protocols.

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Streptavidin HRP conjugates, high sensitivity, Pierce™

Streptavidin HRP conjugates, high sensitivity, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ High Sensitivity Streptavidin-HRP is a peroxidase-conjugated streptavidin biotin-binding protein that provides signal amplification like poly-HRP and exceptional storage stability.

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Iminobiotin agarose, Pierce™

Iminobiotin agarose, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Iminobiotin Agarose is for the purification of avidin, streptavidin or Thermo Scientific NeutrAvidin® Conjugates. Iminobiotin is a cyclic guanido analogue of biotin and has a lower affinity constant for binding avidin, streptavidin or NeutrAvidin® Protein.

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Immobilised jacalin agarose, Pierce™

Immobilised jacalin agarose, Pierce™

Supplier: Thermo Fisher Scientific

Jacalin is an alpha-D-galactose binding lectin extracted from jack-fruit seeds (Artocarpus integrifolia). This lectin is a glycoprotein (approx. 40 kDa) composed of four identical subunits. Jacalin binds to human IgA1 (also called serum or secretory IgA) with high specificity (i.e., it does not bind IgG or IgM). Thus, Jacalin that has been immobilised on crosslinked beaded agarose is useful for purifying human IgA from serum or colostrum samples.

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Immobilised GST agarose, Pierce™

Immobilised GST agarose, Pierce™

Supplier: Thermo Fisher Scientific

Immobilised GST is glutathione S-transferase from Schistosoma japonicum that has been covalently immobilised on crosslinked 6% beaded agarose.

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TCEP solution, neutral pH, Bond-Breaker™ Pierce™

TCEP solution, neutral pH, Bond-Breaker™ Pierce™

Supplier: Thermo Fisher Scientific

Bond-Breaker™ TCEP Solution (neutral pH) is a stable, 0,5 M solution of the thiol-free, phosphine-based TCEP compound, useful as a 10X stock for addition to SDS-PAGE sample loading buffers to reduce protein disulphide bonds.

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Recombinant protein L, biotinylated, Pierce™

Recombinant protein L, biotinylated, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Biotinylated Recombinant Protein L can be used to probe and detect mouse and human antibodies containing kappa light chains in Western blotting, ELISA, IHC and other immunoassay protocols.

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Pierce™ Recombinant Protein G, Biotinylated

Pierce™ Recombinant Protein G, Biotinylated

Supplier: Thermo Fisher Scientific

Biotinylated recombinant Protein G can be used to probe and detect mouse and human antibodies, especially IgG isotypes, in western blotting, ELISA, IHC and other immunoassay protocols.

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Pegylation reagents, Methyl-PEG-NHS-Esters, Pierce™

Pegylation reagents, Methyl-PEG-NHS-Esters, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ MS(PEG)n reagents are methyl-terminated, polyethylene glycol compounds (n equals 4 to 24 PEG units) and TMS(PEG)12 is a three-branched, polyethylene glycol compound (3× 12 PEG units). Both are activated as NHS esters for covalent pegylation of primary amines on proteins (eg. lysines) or assay surfaces.

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Recombinant protein A/G, peroxidase conjugated, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Recombinant Peroxidase Conjugated Protein A/G is useful to probe and detect antibodies, especially IgG isotypes, of many different species hosts in Western blotting, ELISA, IHC and other immunoassay protocols.

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Recombinant protein A/G, alkaline phosphatase conjugated, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Recombinant Alkaline Phosphatase Conjugated Protein A/G is useful to probe and detect antibodies, especially IgG isotypes, of many different species hosts in Western blotting, ELISA, IHC and other immunoassay protocols.

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