Reagenti per estrazione di proteine
I reagenti per la purificazione delle proteine vengono utilizzati per isolare una o più proteine da una miscela complessa, solitamente da cellule, tessuti o organismi interi. Questo processo è fondamentale per la caratterizzazione della funzione e della struttura, nonché per identificare le interazioni della proteina di interesse. Durante le varie fasi, i reagenti per la purificazione delle proteine assistono nella lisi cellulare, nella separazione delle componenti proteiche solubili dai detriti cellulari e infine nella separazione della proteina di interesse dalle impurità legate al prodotto e al processo.
Recombinant protein L, biotinylated, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Biotinylated Recombinant Protein L can be used to probe and detect mouse and human antibodies containing kappa light chains in Western blotting, ELISA, IHC and other immunoassay protocols.
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Pegylation reagents, Methyl-PEG-NHS-Esters, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ MS(PEG)n reagents are methyl-terminated, polyethylene glycol compounds (n equals 4 to 24 PEG units) and TMS(PEG)12 is a three-branched, polyethylene glycol compound (3× 12 PEG units). Both are activated as NHS esters for covalent pegylation of primary amines on proteins (eg. lysines) or assay surfaces.
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Pierce™ Recombinant Protein G, Biotinylated
Supplier: Thermo Fisher Scientific
Biotinylated recombinant Protein G can be used to probe and detect mouse and human antibodies, especially IgG isotypes, in western blotting, ELISA, IHC and other immunoassay protocols.
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Recombinant protein A/G, peroxidase conjugated, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Recombinant Peroxidase Conjugated Protein A/G is useful to probe and detect antibodies, especially IgG isotypes, of many different species hosts in Western blotting, ELISA, IHC and other immunoassay protocols.
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Immobilised TCEP disulphide reducing gel, Pierce™
Supplier: Thermo Fisher Scientific
Immobilised TCEP disulphide Reducing Gel eliminates the need to use laborious and inefficient gel filtration methods to separate the reduced sample from the reducing agent.
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PEGylated N-succinimidyl S-acetylthioacetate, SAT(PEG)4, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ SAT(PEG)₄ (PEGylated N-succinimidyl S-acetylthioacetate) is a sulphhydryl-addition reagent for covalent modification of primary amines.
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Protein stabilising cocktail, Pierce™
Supplier: Thermo Fisher Scientific
The Protein Stabilising Cocktail is a versatile stabilising solution that increases the shelf-life of purified or partially purified proteins during routine storage.
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Inclusion body solubilisation reagent, Pierce™
Supplier: Thermo Fisher Scientific
Inclusion Body Solubilisation Reagent is designed to retrieve expressed protein in soluble form after lysis and extraction with reagents.
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Recombinant protein G, peroxidase conjugated, Calbiochem®
Supplier: Merck Millipore (Oncogene)
Purified recombinant Protein G conjugated to peroxidase can be used in direct immunoassay.
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Recombinant streptavidin
Supplier: G-Biosciences
Recombinant streptavidin from Streptomyces avidinii, produced in E.coli.
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BTSNM support materials, beads, 0,4 g with buffer
Supplier: G-Biosciences
Beads are made of a styrene divinylbenzene copolymer containing paired iminodiacetate ions, which act as chelating groups in binding polyvalent metal ions.
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HCCA matrix
Supplier: BRUKER DALTRONIK
Pre-portioned HCCA matrix provides easy and convenient preparation of HCCA matrix solutions.
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Pegylation reagent, CT(PEG)₁₂ carboxy-PEG-thiol compound
Supplier: Thermo Fisher Scientific
Carboxy-PEG12-thiol, is a carboxyl- and sulphhydryl-terminated compound that contains a 12-unit polyethylene glycol (PEG) spacer and is used to modify surfaces such as quantum dots, monolayers and magnetic particles.
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Coupling catalyst, GlycoLink™
Supplier: Thermo Fisher Scientific
An optimized buffer for aldehyde-hydrazide and aldehyde-alkoxyamine coupling reactions.
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Mass spectrometry reagents, MALDI matrices, single-use
Supplier: Thermo Fisher Scientific
MALDI Matrices are highly purified, recrystallised reagents packaged in convenient single-use tubes sufficient for analysing up to 96 samples.
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Pegylation reagents, methyl-PEG-amine compounds
Supplier: Thermo Fisher Scientific
The methyl-PEGn-amine (MA[PEG]n) PEGylation reagents are methyl ether-terminated PEG amines that are used for modifying proteins or surfaces such as beads, nanoparticles and self-assembled monolayers. These PEGylation reagents are homogenous compounds of defined molecular weight and spacer length, providing precision in optimising modification applications.



