I reagenti per acidi nucleici costituiscono la base dell'analisi genetica e della biologia molecolare, svolgendo un ruolo cruciale dalla clonazione agli studi di espressione genica. Esplora la nostra collezione di enzimi ad alta fedeltà per PCR di fine punto, reagenti avanzati per l'amplificazione isoterma e kit completi per la purificazione degli acidi nucleici. Per una precisione nei saggi quantitativi, esplora la nostra selezione di kit qPCR e RT-qPCR, ottimizzati per un'accuratezza senza pari. Il nostro portafoglio include anche reagenti di sequenziamento di nuova generazione, offrendo soluzioni all'avanguardia per il sequenziamento genomico e i progressi nella medicina personalizzata.
T4 DNA ligase
Supplier: Cytiva
T4 DNA ligase catalyses the formation of a phosphodiester bond between the 5'-phosphoryl group and the 3'-hydroxyl group of two double-stranded DNA fragments. ATP is required for this reaction.
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Recombinant Taq DNA Polymerase
Supplier: Cytiva
For PCR and other procedures requiring DNA polymerase activity at elevated temperatures.
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Miscele qPCR, ABsolute Blue™
Supplier: Thermo Fisher Scientific
Il Master Mix ABsolute Blue™ contiene un colorante blu inerte per migliorare significativamente il contrasto tra il reagente e la plastica, rendendo la verifica dell'erogazione del Master Mix rapida, semplice e a prova di errore. Le applicazioni includono l'espressione del gene, la validazione siRNA, il tipo SNP e il genotipo.
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Kit RT-PCR, Verso™
Supplier: Thermo Fisher Scientific
Il sistema RT-PCR Verso™ unisce un enzima di trascrizione inversa, opzioni di innesco migliorate e tamponi ottimizzati per generare cDNA di lunghezza intera e ad alta resa. Con un'ampia varietà di opzioni kit per le reazioni RT-PCR mono e bifase, il sistema Verso™ garantisce flessibilità e sensibilità ottimali in un formato facile da usare.
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Biglie per PCR, illustra™ PuReTaq Ready-To-Go™
Supplier: Cytiva
PuReTaq RTG PCR beads and reagents ensures reliable and robust performance in both end point and RT-PCR amplifications and ensures the lowest possible levels of contaminating prokaryotic and eukaryotic nucleic acids.
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illustra™ Hot Start master mix
Supplier: Cytiva
illustra™ Hot Start Master Mix is a 2X premixed formulation that can effectively reduce non specific priming and primer-dimer formation during PCR. illustra™ Hot Start Master Mix uses a novel hot start method called primer sequestration.
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Real Time PCR Detection Test Kits, Supreme HLVd (Hop Latent Viroid)
Supplier: BIOPREMIER
SUPREME RT-PCR detection kit HLVd is designed for the detection of Hop Latent Viroid (HLVd) in a single PCR reaction. The kit is suitable for use in real time instruments with FAM and Cy5 detection channels.
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oasig™ Lyophilised master mixes
Supplier: PRIMERDESIGN
The oasig freeze drying process stabilises all of the active components allowing these reagents to be shipped and stored at room temperature. This hugely simplifies the logistics of purchasing, shipping and using the technology. Whether you are in a sophisticated laboratory in Texas or a mobile field hospital in Timbuktu we can supply complete qPCR kit and reagent packages to your door quickly and cheaply via standard shipping methods without the need for dry ice or a cold chain of any sort.
Freeze dried qPCR reagents stored and shipped at ambient temperatures:
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illustra™ Hot Start mix, Ready-To-Go™
Supplier: Cytiva
illustra™ Hot Start Mix, Ready-To-Go™ is a pre-formulated and pre-dispensed, freeze-dried PCR reagent mix, including high quality PuReTaq™ DNA polymerase and a hot start activator protein, for increased specificity and reproducibility of PCR amplifications. The Ready-To-Go™ beads are provided in either 0,5 or 0,2 ml tubes that are compatible with most thermal cyclers.
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ABsolute™ qPCR Low ROX Mixes
Supplier: Thermo Fisher Scientific
ABsolute™ qPCR low ROX mixes are optimised for use with real time instruments that are able to detect long wavelength dyes with greater sensitivity, such as the ABI 7500. The lower level of ROX still facilitates normalisation across the plate, while maintaining the sensitivity of the assay. The mixes contain all the components necessary to perform quantitative PCR, with the exception of template and primers/probes. The 2X qPCR mix contains a proprietary reaction buffer that provides high sensitivity, specificity and reproducibility.
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Kit qRT-PCR, Verso™
Supplier: Thermo Fisher Scientific
Il sistema qRT-PCR Verso™ combina l'enzima RT Verso™ e il Master Mix ABsolute™ Blue qPCR per garantire un prodotto estremamente economico e versatile. Disponibile in formato mono o bifase, in confezioni multiple, per adattarsi ai requisiti di ogni applicazione e resa.
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ABsolute™ qPCR Capillary Mixes
Supplier: Thermo Fisher Scientific
ABsolute™ qPCR Capillary Mixes contain an optimised reaction buffer specifically designed to provide the highest possible sensitivity, specificity and consistent end-point values in real time instruments that use glass capillaries, such as the Roche LightCycler®. The 2X mixes contain all the components necessary to perform quantitative PCR, with the exception of template and primers/probes.
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ABsolute™ qPCR Mixes
Supplier: Thermo Fisher Scientific
ABsolute™ qPCR Mixes contain all the components necessary to perform quantitative PCR, with the exception of template and primers. Versions are available for use with probes or with SYBR® Green I included, and with or without ROX or Fluorescein passive reference dyes.
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Real Time PCR Detection Test Kits, Supreme HLVd/LCV/CanCv
Supplier: BIOPREMIER
This Supreme RT-PCR detection kit HLVd/LCV/CanCV is designed for the detection of Hop Latent Viroid (HLVd), Lettuce Chlorotic Virus (LCV) and Cannabis Cryptic Virus (CanCV) in a single PCR reaction (TETRAPLEX).
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genesig® Easy DNA qPCR Detection Kit (Salmonella spp)
Supplier: PRIMERDESIGN
This genesig® Easy qPCR detection kit targets the invasion protein (invA) gene from Salmonella spp. The kit is designed for the in vitro quantification of Salmonella spp genomes and to have a broad detection profile.
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GenFind V3 Reagent Kits
Supplier: Beckman Coulter
This prep system kit isolates and purifies genomic DNA (gDNA) from whole blood. The kit is uses Solid Phase Reversible Immobilisation (SPRI) paramagnetic bead-based technology to effectively produce a high recovery of high quality DNA for downstream applications such as PCR.
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DirectPCR® DNA extraction system
Supplier: VIAGEN BIOTECH
DirectPCR® DNA Lysis Reagents offer a quick and cost-effective 'single-tube' system for the rapid preparation of PCR template DNA from mouse tails, ears, embryonic material or mammalian cells.
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Gel and PCR Clean-up, NucleoSpin®
Supplier: MACHEREY-NAGEL
NucleoSpin® Gel and PCR Clean-up kits allow 2-in-1 clean up with optimised recovery and elution volume.
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hPSC Genetic Analysis Kit
Supplier: STEMCELL Technologies
qPCR analysis kit for detecting the majority of karyotypic abnormalities reported in human ES and iPS cells.
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qPCR Master Mix Kit
Supplier: STEMCELL Technologies
For probe-based assays and arrays.