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Hai cercato: Reagenti per acidi nucleici

Reagenti per acidi nucleici

I reagenti per acidi nucleici costituiscono la base dell'analisi genetica e della biologia molecolare, svolgendo un ruolo cruciale dalla clonazione agli studi di espressione genica. Esplora la nostra collezione di enzimi ad alta fedeltà per PCR di fine punto, reagenti avanzati per l'amplificazione isoterma e kit completi per la purificazione degli acidi nucleici. Per una precisione nei saggi quantitativi, esplora la nostra selezione di kit qPCR e RT-qPCR, ottimizzati per un'accuratezza senza pari. Il nostro portafoglio include anche reagenti di sequenziamento di nuova generazione, offrendo soluzioni all'avanguardia per il sequenziamento genomico e i progressi nella medicina personalizzata.

5-Tetramethylrhodamine-dUTP, 1 mM Solution

Supplier: Biotium

5-TAMRA-dUTP (5-Tetramethylrhodamine-dUTP) can be enzymatically incorporated into DNA to make fluorescently labeled probes, and has absorption/emission at 553/577 nm. Note: 5-TAMRA-dUTP may not be compatible with PCR labeling of DNA probes. Supplied as a 1 mM solution in Tris-HCl buffer, pH 7.5.

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TriLink® 2'-Deoxycytidine-5'-Triphosphate

TriLink® 2'-Deoxycytidine-5'-Triphosphate

Supplier: TriLink BioTechnologies

This nucleotide consists of an unmodified base, a deoxyribose, and a 5′ triphosphate group for synthesis of DNA molecules.

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Fluorescent nucleotides, Red 594 dUTP and Red 598 dUTP

Supplier: ENZO LIFE SCIENCES

Red dUTP's (Red 594 dUTP, Red 598 dUTP) can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase.

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TriLink® 5-Methyluridine-5'-Triphosphate

TriLink® 5-Methyluridine-5'-Triphosphate

Supplier: TriLink BioTechnologies

TriLink® 5-Methyluridine-5'-Triphosphate

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TriLink® CleanAmp® 7-Deaza-dGTP

TriLink® CleanAmp® 7-Deaza-dGTP

Supplier: TriLink BioTechnologies

CleanAmp® dNTPs are chemically-modified nucleotides designed to improve specificity, sensitivity, and robustness of your PCR.

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TriLink® N1-Ethylpseudouridine-5'-Triphosphate

TriLink® N1-Ethylpseudouridine-5'-Triphosphate

Supplier: TriLink BioTechnologies

A modified NTP for incorporation into messenger RNAs (mRNA) using T7 RNA Polymerase. Incorporation of N1-ethylpseudouridine can reduce the immunogenicity of the resulting mRNA.

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TriLink® 2'-Fluoro-2'-deoxyuridine-5'-Triphosphate

TriLink® 2'-Fluoro-2'-deoxyuridine-5'-Triphosphate

Supplier: TriLink BioTechnologies

2'-Fluoro NTPs are being utilised in an increasing number of applications in research and new drug development. 2'-Fluoro-dUTP is incorporated in both DNA and RNA constructs to improve in vivo stability.

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TriLink® N1-Methoxymethylpseudouridine-5'-Triphosphate

TriLink® N1-Methoxymethylpseudouridine-5'-Triphosphate

Supplier: TriLink BioTechnologies

TriLink® N1-Methoxymethylpseudouridine-5'-Triphosphate

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Peqlab peqGOLD, RNA Isolation, Plant RNA Kit

Peqlab peqGOLD, RNA Isolation, Plant RNA Kit

Supplier: VWR Chemicals

Plant RNA Kit efficiently isolates RNA from a wide range of plant species and plant tissue, effectively removing genomic DNA, polysaccharides, phenol and other secondary metabolites. Starting amount can be up to 100 mg plant tissue. The isolated RNA is directly suitable for downstream applications.

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Peqlab peqGOLD, DNA Isolation, Bacterial DNA Mini Kit

Peqlab peqGOLD, DNA Isolation, Bacterial DNA Mini Kit

Supplier: VWR Chemicals

Bacterial DNA Mini Kit for the extraction of high quality DNA from gram-positive and gram-negative bacteria. For up to 1×10⁹ cells per preparation typical yield is up to 30 µg.

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Peqlab peqGOLD, DNA Clean-up, Gel Extraction Kits

Peqlab peqGOLD, DNA Clean-up, Gel Extraction Kits

Supplier: VWR Chemicals

Purifies DNA bands 70 bp to 20 kb in length from agarose gels. The DNA band of interest is excised from the gel, dissolved in binding buffer, and efficiently processed in a peqGOLD DNA column. >90% recovery. Elution volume: 30 to 50 µl.

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Peqlab peqGOLD TriFast™, DNA/RNA/protein purification reagent

Peqlab peqGOLD TriFast™, DNA/RNA/protein purification reagent

Supplier: VWR Chemicals

TriFast™ is a complete ready to use reagent for simultaneous isolation of RNA, DNA and proteins from liquid samples, tissue and cells.

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VWR®, DNA extraction Kit for Real-time PCR food samples analysis

VWR®, DNA extraction Kit for Real-time PCR food samples analysis

Supplier: VWR Chemicals

Rapid method for DNA extraction and purification from all types of microorganisms in a variety of matrices for molecular biology.

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Peqlab peqGOLD, DNA Isolation, Plant DNA Mini Kit

Peqlab peqGOLD, DNA Isolation, Plant DNA Mini Kit

Supplier: VWR Chemicals

Dependable isolation of high quality total cellular DNA from a variety of plant species and tissues. peqGOLD homogeniser columns remove cell debris and improve sample handling following lysis.

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VWR® Peqlab peqGOLD Plasmid Purification, Plasmid Miniprep Kit I

VWR® Peqlab peqGOLD Plasmid Purification, Plasmid Miniprep Kit I

Supplier: VWR Chemicals

Plasmid Miniprep Kit I for the easy, quick and efficient extraction of highly pure plasmid DNA. Isolate up to 30 µg of high quality plasmid DNA from 1 to 5 ml bacterial cultures in 30 minutes or less. Plasmid DNA obtained with peqGOLD Plasmid Miniprep kits can directly be used for a wide range of downstream applications.

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Peqlab peqGOLD, DNase I Digest Kit

Peqlab peqGOLD, DNase I Digest Kit

Supplier: VWR Chemicals

The peqGOLD DNase I Digest Kit is optimised for use with peqGOLD RNA extraction protocols. Normally DNase I digestion is not required for RNA purified with peqGOLD RNA Mini Columns as our silica-based spin column technology efficiently removes the majority of DNA without enzymatic digestion. However, certain sensitive RNA applications may require further DNA removal.

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Peqlab peqGOLD, Plasmid Purification, Plasmid Miniprep Kit II

Peqlab peqGOLD, Plasmid Purification, Plasmid Miniprep Kit II

Supplier: VWR Chemicals

Isolate up to 40 to 70 µg of high quality plasmid DNA from up to 15 ml bacterial cultures in 30 minutes or less. Simplified with VWR® peqGold Mini Column II technology into three quick steps: Bind, wash and elute.

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Peqlab peqGOLD Cycle-Pure™, PCR Clean-up, Kits

Peqlab peqGOLD Cycle-Pure™, PCR Clean-up, Kits

Supplier: VWR Chemicals

Purification of single- or double-stranded DNA from PCR or other enzymatic reactions.

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Peqlab peqGOLD, DNA Isolation, Microspin Tissue DNA Mini Kits

Peqlab peqGOLD, DNA Isolation, Microspin Tissue DNA Mini Kits

Supplier: VWR Chemicals

Fast, easy, and economical kit for isolation of genomic DNA from small sample volumes and sizes for consistent PCR and analytical test results.

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VWR®, Phenol-free total RNA purification kit

VWR®, Phenol-free total RNA purification kit

Supplier: VWR Chemicals

The phenol-free total RNA purification kit provides a rapid method for the isolation and purification of total RNA from cultured animal cells, tissue samples, blood, bacteria, yeast, fungi, plants and viruses.

    
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TriLink® CleanAmp® 7-deaza-dGTP Mix

TriLink® CleanAmp® 7-deaza-dGTP Mix

Supplier: TriLink BioTechnologies

CleanAmp® dNTPs are chemically-modified nucleotides designed to improve specificity, sensitivity, and robustness of your PCR.

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TriLink® 7-Deaza-7-Propargylamino-2'-deoxyadenosine-5'-Triphosphate

TriLink® 7-Deaza-7-Propargylamino-2'-deoxyadenosine-5'-Triphosphate

Supplier: TriLink BioTechnologies

TriLink® 7-Deaza-7-Propargylamino-2'-deoxyadenosine-5'-Triphosphate

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TriLink® 2'-Deoxyadenosine-5'-Triphosphate

TriLink® 2'-Deoxyadenosine-5'-Triphosphate

Supplier: TriLink BioTechnologies

This nucleotide consists of an unmodified base, a deoxyribose, and a 5′ triphosphate group for synthesis of DNA molecules.

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TriLink® CleanAmp® dNTP Mix

TriLink® CleanAmp® dNTP Mix

Supplier: TriLink BioTechnologies

CleanAmp® dNTPs are chemically-modified nucleotides designed to improve specificity, sensitivity, and robustness of your PCR.

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Cyanine 3-UTP (enhanced)

Cyanine 3-UTP (enhanced)

Supplier: ENZO LIFE SCIENCES

Cyanine 3-UTP (Enhanced) can replace UTP as a substrate for T7 RNA polymerase in labeling systems that generate labeled probes through in vitro transcription. Probes generated by these methods are suitable for multicolor fluorescence analysis, specifically dual-color expression arrays in conjunction with cyanine 3-UTP .

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Cyanine 5-dUTP

Supplier: ENZO LIFE SCIENCES

Cyanine 5-dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labeled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, cDNA labeling and 3’-end labeling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Cyanine 5-dUTP can also be used for multicolor fluorescence labeling.

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VWR® ExoCleanUp FAST, One-step PCR Clean-up

VWR® ExoCleanUp FAST, One-step PCR Clean-up

Supplier: VWR Chemicals

VWR® ExoCleanUp FAST PCR reagent is a one-step PCR clean-up reagent for optimal sequencing results, consisting of a balanced combination of a heat-labile exonuclease I (HL-ExoI) and a recombinant shrimp alkaline phosphatase (rSAP).

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Peqlab peqGOLD, DNA Isolation, Blood And Tissue DNA Mini Kits

Peqlab peqGOLD, DNA Isolation, Blood And Tissue DNA Mini Kits

Supplier: VWR Chemicals

Fast, easy, and economical kit for isolation of DNA from a variety of samples including fresh or frozen cultured cells, tissue, blood, buccal swabs and saliva.

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E.Z.N.A.® Plant & Fungal DNA Kit

E.Z.N.A.® Plant & Fungal DNA Kit

Supplier: OMEGA BIO-TEK

Isolate DNA from plant or fungal samples using spin columns.

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E.Z.N.A.® Gel extraction kits

E.Z.N.A.® Gel extraction kits

Supplier: OMEGA BIO-TEK

La purificazione di DNA da gel è una tecnica comune utilizzata per isolare specifici frammenti di DNA. Tuttavia, la maggior parte dei metodi può non eliminare del tutto l'agarosio (con conseguenti problemi nelle applicazioni successive), può troncare il DNA o causare rese molto basse.

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