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Reagenti per acidi nucleici

Reagenti per acidi nucleici

I reagenti per acidi nucleici costituiscono la base dell’analisi genetica e della biologia molecolare, svolgendo un ruolo fondamentale dalla clonazione agli studi sull’espressione genica. Esplora la nostra collezione con enzimi ad alta fedeltà per PCR end-point, reagenti avanzati per l’amplificazione isotermica e kit completi per la purificazione degli acidi nucleici. Per una precisione nelle analisi quantitative, scopri la nostra selezione di kit qPCR e RT-qPCR, progettati per un’accuratezza senza eguali. Il nostro portafoglio comprende anche reagenti per il sequenziamento di nuova generazione, che offrono soluzioni all’avanguardia per il sequenziamento genomico e i progressi nella medicina personalizzata.

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Multipurpose Mini Spin Columns, Abcam

Supplier: Abcam

This product is manufactured by BioVision, an Abcam company and was previously called 6572 Multipurpose Mini Spin Columns.

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Mutagenesis kits QuikChange

Mutagenesis kits QuikChange

Supplier: AGILENT

The original QuikChange Site-Directed Mutagenesis Kits speed up and simplify site-directed mutagenesis studies. The kits eliminate the need for sub-cloning into M13-based bacteriophage vectors and for ss-DNA rescue. This allows oligo-mediated introduction of site-specific mutations into virtually any double-stranded plasmid DNA. In addition, the XL version of the kit is specially designed for efficient mutagenesis of large (4 to 14 kb) or otherwise difficult-to mutagenise plasmid templates. The XL kit features components specifically designed for more efficient DNA replication and bacterial transformation.

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AxyPrep™ FragmentSelect-I kit, Axygen®

AxyPrep™ FragmentSelect-I kit, Axygen®

Supplier: Corning

The AxyPrep™ FragmentSelect-I Kit is optimized for Illumina next generation sequencing platforms and Life Technologies (SOLiD) fragment size selection needs to simplify the library construction workflow. The kit is designed for fragment size selection needs in the Roche/454’s DNA Genome sequencer library construction workflow.

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AMPIGENE® qPCR 1-Step Probe Kit Hi-ROX

Supplier: Enzo Life Sciences

Optimised and efficient cDNA synthesis and probe-based qPCR in a single tube.

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Brilliant II probe MM

Supplier: AGILENT

Brilliant II QPCR and QRT-PCR reagents were developed for improved sensitivity of detection, ensuring reproducible quantification even at low target concentrations.

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High-Sensitivity DNA Library Preparation Kit (For Illumina®), Abcam

Supplier: Abcam

High Sensitivity DNA Library Preparation KIt (ab185905) is a complete set of optimized reagents to prepare a DNA library from very small amount of samples for use in next-generation sequencing applications.

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PCR cloning kit, CloneJET

PCR cloning kit, CloneJET

Supplier: Thermo Fisher Scientific

The CloneJET PCR cloning kit contains a ready-to-use positive selection cloning vector pJET1.2/blunt. The vector contains a lethal restriction enzyme gene that is disrupted by ligation of a DNA insert into the cloning site. As a result, only bacterial cells with recombinant plasmids are able to form colonies. Recircularised pJET1.2/blunt vector molecules lacking an insert express a lethal restriction enzyme, which kills the host E. coli cell after transformation. This positive selection drastically accelerates the process of colony screening and eliminates additional costs required for blue/white selection.

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Brilliant II SYBR® Master mix

Supplier: AGILENT

Brilliant II SYBR® QPCR and QRT-PCR reagents were developed for improved sensitivity of detection, ensuring reproducible quantification even at low target concentrations.

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Mag-Bind® stool DNA 96 kit

Supplier: OMEGA BIO-TEK

The Mag-Bind® Stool DNA 96 Kit allows rapid and reliable isolation of high-quality genomic DNA (both pathogen and host DNA) from stool samples.

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Absolutely total RNA purification kits

Supplier: AGILENT

Absolutely RNA Product portfolio makes RNA purification from tissue and cell samples easy and fast.

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Restriction enzymes, BamHI, HC, Fermentas

Restriction enzymes, BamHI, HC, Fermentas

Supplier: Thermo Fisher Scientific

GATCC sites and cuts best at 37 °C in its own unique buffer.

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qPCR SuperMix, PerfeCTa® MultiPlex

Supplier: Quantabio

La miscela SuperMix con concentrazione 2X è un assortimento di reazioni pronte all'uso e contiene tutti i componenti necessari eccetto i primer, le sonde e i templati. Questo sistema consente di superare i molteplici limiti delle miscele Master per PCR tradizionali, consentendo l'amplificazione non affetta da errore sistematico di fino a cinque sequenze target in un'unica provetta. L'occultamento di ampliconi a basso numero di copie da parte di target di riferimento con numero di copie più elevato durante l'amplificazione è un problema comune nella PCR multiplex. Ciò può deviare o mascherare la rappresentazione e la quantificazione delle sequenze target a basso numero di copie. PerfeCTa® MultiPlex qPCR SuperMix offre una' elevata sensibilità paragonabile a quelle dei saggi mediante sonda in qPCR singleplex senza dover limitare o variare le concentrazioni dei primer.

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Restriction enzymes, AgeI, FastDigest™, Fermentas

Restriction enzymes, AgeI, FastDigest™, Fermentas

Supplier: Thermo Fisher Scientific

Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

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Mag-Bind® FFPE DNA/RNA 96 Kit

Mag-Bind® FFPE DNA/RNA 96 Kit

Supplier: OMEGA BIO-TEK

Sequentially isolate DNA and RNA in two separate eluates from the same FFPE sample.

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E.Z.N.A.® Formalin-Fixed, Paraffin-Embedded (FFPE) Tissue DNA Kits

Supplier: OMEGA BIO-TEK

Kit di estrazione basato sull'utilizzo di proteasi selezionate per l'estrazione di microgrammi di DNA da campioni in FFPE. Gli acidi nucleici purificati, sebbene estremamente frammentati, sono adatti per numerose analisi di espressione genica e sequenziamento genomico. I kit con colonnine da centrifuga E.Z.N.A® sono ideali per applicazioni a bassa resa, mentre i kit con biglie magnetiche Mag-Bind® sono progettati appositamente per applicazioni a resa elevata e con possibilità di automazione.

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cDNA Synthesis Kit

Supplier: OriGene

qSTAR first-strand cDNA synthesis kit for miRNA is a two-step protocol kit.

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