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Reagenti per acidi nucleici

Reagenti per acidi nucleici

I reagenti per acidi nucleici costituiscono la base dell’analisi genetica e della biologia molecolare, svolgendo un ruolo fondamentale dalla clonazione agli studi sull’espressione genica. Esplora la nostra collezione con enzimi ad alta fedeltà per PCR end-point, reagenti avanzati per l’amplificazione isotermica e kit completi per la purificazione degli acidi nucleici. Per una precisione nelle analisi quantitative, scopri la nostra selezione di kit qPCR e RT-qPCR, progettati per un’accuratezza senza eguali. Il nostro portafoglio comprende anche reagenti per il sequenziamento di nuova generazione, che offrono soluzioni all’avanguardia per il sequenziamento genomico e i progressi nella medicina personalizzata.

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Mag-Bind® Plant DNA DS 96 kit

Supplier: OMEGA BIO-TEK

The Mag-Bind® Plant DNA DS 96 Kit is designed for difficult samples (DS) which are hard to lyse or contain high amounts of polysaccharides and polyphenols. The system uses a CTAB-based lysis buffer which does not require organic solvents. The proprietary binding system prevents inhibitors from binding to the magnetic beads. The wash buffer system further removes polysaccharides, phenolic compounds and enzyme inhibitors from plant tissue lysate. An optional rebinding step is included with the kit but most samples do not require the additional treatment.

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TriLink® 2'-Deoxythymidine-5'-Triphosphate

TriLink® 2'-Deoxythymidine-5'-Triphosphate

Supplier: TriLink BioTechnologies

This nucleotide consists of an unmodified base, a deoxyribose, and a 5′ triphosphate group for synthesis of DNA molecules.

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DNA Library Prep Kit, sparQ

DNA Library Prep Kit, sparQ

Supplier: Quantabio

An optimised kit for the rapid construction of DNA libraries from fragmented double-stranded DNA for sequencing on Illumina® NGS platforms and prepares for sequencing success with the highest quality library.

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TriLink® Guanosine-5'-Triphosphate

TriLink® Guanosine-5'-Triphosphate

Supplier: TriLink BioTechnologies

This nucleotide consists of an unmodified base, a ribose, and a 5′ triphosphate group for synthesis of RNA molecules.

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TriLink® 2',3'-Dideoxyadenosine-5'-O-(1-Thiotriphosphate)

TriLink® 2',3'-Dideoxyadenosine-5'-O-(1-Thiotriphosphate)

Supplier: TriLink BioTechnologies

2',3'-Dideoxyadenosine-5'-O-(1-Thiotriphosphate) is a sugar modified nucleoside triphosphate where the 2' and 3' hydroxyl groups are absent, resulting in chain termination. This ddATP analog also contains a 1-Thiotriphosphate which results in a nuclease resistant phosphorothioate linkage. The inability of polymerases to extend from a dideoxy nucleotide causes chain termination and is useful in antiviral research and in a variety of biotechnology applications.

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EXO- PFU DNA POLYMERASE 250U 1 * 1 pezzi

Supplier: AGILENT

EXO- PFU DNA POLYMERASE 250U 1 * 1 pezzi

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Mitochondria/Cytosol Fractionation Kit, Abcam

Supplier: Abcam

Mitochondria/cytosol fractionation kit provides a unique formulation of reagents for the effective isolation of highly enriched mitochondrial fractions from the cytosolic fraction of mammalian cells, including both apoptotic and non-apoptotic cells. - Cited in over 110 publications - Isolates mitochondrial and cytosolic fractions for studying apoptotic pathways and signal transduction - Simple and easy procedure for fractionation - Suitable for detecting translocation of factors between fractions via Western blotting (WB), ELISA, or other assays

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E-Z 96® Tissue DNA Kit

E-Z 96® Tissue DNA Kit

Supplier: OMEGA BIO-TEK

Adattando la tecnologia HiBind® alle piastre a 96 pozzetti, il kit E-Z 96® Tissue DNA offre un metodo a resa elevata per la purificazione di DNA genomico da sangue intero, tamponi orali, code di topo, code di ratto, tessuti e cellule animali nel formato a piastra da 96 pozzetti. Il DNA purificato è adatto per la maggior parte delle applicazioni downstream quali PCR, digestione con enzimi di restrizione e ibridazioni.

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qPCR master mixes, Fast Plus EvaGreen®

qPCR master mixes, Fast Plus EvaGreen®

Supplier: Biotium

A qPCR master mix containing Evagreen® qPCR dye and Cheetah™ Taq hotstart DNA polymerase, suitable for qPCR using a fast cycling protocol.

   Opzioni sostenibili disponibili
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T7 RNA polymerase

Supplier: Thermo Fisher Scientific

T7 RNA polymerase is ideal for synthesis of unlabeled and labeled RNA. It catalyses the 5'→3' synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from it promoter. It is also used in the study of RNA secondary structure and RNA-protein interactions, RNA splicing.

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AMPIGENE® qPCR 1-Step Probe Kit Lo-ROX

Supplier: Enzo Life Sciences

Optimised and efficient cDNA synthesis and probe-based qPCR in a single tube.

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Restriction enzymes, NcoI, FastDigest™, fermentas

Restriction enzymes, NcoI, FastDigest™, fermentas

Supplier: Thermo Fisher Scientific

Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

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Montage® SEQ96 Sequencing Reaction Cleanup Kits

Montage® SEQ96 Sequencing Reaction Cleanup Kits

Supplier: Merck

The Montage® SEQ96 sequencing reaction cleanup kit provides the filtration plate and solution necessary to remove contaminating salts and unincorporated dye terminators from DNA sequencing reactions.

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Restriction enzymes, NotI, 50 U/µl, Fermentas

Restriction enzymes, NotI, 50 U/µl, Fermentas

Supplier: Thermo Fisher Scientific

GGCCGC sites and cuts best at 37 °C in O buffer.

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TriLink® Engineered RNase Inhibitor

TriLink® Engineered RNase Inhibitor

Supplier: TriLink BioTechnologies

Engineered RNase Inhibitor is a protein to inhibit RNase activities to prevent RNA degradation in IVT.

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PerfeCTa® PreAmp 5X SuperMix

PerfeCTa® PreAmp 5X SuperMix

Supplier: Quantabio

PerfeCTa® PreAmp SuperMix is a 5X concentrated, ready to use reaction cocktail for unbiased, selected enrichment of target sequences from limited amounts of starting material for downstream gene expression profiling or targeted resequencing. It contains all components, except primers and templates. The 5X concentrated SuperMix allows addition of higher template volumes when working with low concentration samples, and/or reduced reaction volumes. Inclusion of an inert light blue tracer dye helps visualise small reaction volumes and ensure accurate pipetting.

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