Tissue culture buffers provide an energy source, maintain proper pH levels, and keep ideal osmotic pressure present. Used predominately in biomolecular research, the dry and liquid substances have a balanced inorganic salt composition. Lower concentration options permit equilibration with air in a closed system, while higher salt concentration options keep gaseous phases in equilibrium. Cell growth is optimal when plated in a nutritional medium that has been buffered.
Phosphate-buffered saline (PBS, 10X), pH 7,6
Supplier: Thermo Fisher Scientific
Cell culture buffer, PBS, 10 X
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TE buffer, pH 8,1, RNAse free
Supplier: Thermo Fisher Scientific
Trizma is used in the formulation of buffer solutions in the pH range between 7,5 and 8,5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification.
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Krebs-Ringer solution, bicarbonate-buffered
Supplier: Thermo Fisher Scientific
Contains: 120 mM sodium chloride, 5 mM potassium chloride, 2 mM calcium chloride, 1 mM magnesium chloride, 25 mM sodium bicarbonate, and 5,5 mM D-glucose.
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Sodium bicarbonate, Corning®
Supplier: Corning
Sodium bicarbonate is used as part of a buffering system commonly used to maintain physiological pH 7,2 to 7,4 in a culture environment
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HEPES Buffer, ES Qualified, HyClone™ AdvanceSTEM™
Supplier: HyClone products (Cytiva)
HyClone AdvanceSTEM ES Qualified HEPES provides maintenance of physiological pH in cell culture despite changes in carbon dioxide.
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HEPES-buffered saline, pH 7,0 (2X for transfection)
Supplier: Thermo Fisher Scientific
Cell culture buffer, HEPES-buffered Saline
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Phosphate-buffered saline (DPBS, 10X), Dulbecco's formula
Supplier: Thermo Fisher Scientific
Cell culture buffer, Phosphate-buffered saline (DPBS, 10X), Dulbecco's formula
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10% SDS (Sodium Dodecyl Sulfate), Corning®
Supplier: Corning
Sodium Dodecyl Sulfate (SDS) is an anionic detergent with applications in SDS-PAGE protein analysis and nucleic acid purification. It is a wetting agent that is effective in both acid and alkaline solutions, and is most often used as a protein and lipid solubilization reagent. SDS is also a powerful protein denaturant. This 10% solution is prepared in 18 megaohm water and is 0.2µm filtered.
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Carbonate-buffered saline (5X), pH 9,5
Supplier: Thermo Fisher Scientific
Cell culture buffer, Carbonate-buffered Salin, 5X
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Phosphate buffered saline, PBS-TWEEN® tablets, Millipore®
Supplier: Merck Millipore (Oncogene)
Developed for use in immunoassay applications.
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Sodium acetate, Corning® 3M™
Supplier: Corning
Sodium Acetate is a common molecular biology reagent. All production lots are tested to ensure that they are free of RNAse. It is often used in buffer systems to maintain the pH of a given solution.
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Buffer, HEPES solution (1 mol/L), Corning®
Supplier: Corning
HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulphonic acid) provides an alternative to the use of bicarbonate for buffering because it does not require elevated levels of CO2
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Hanks' Balanced Salts (Modified), 1X, without Magnesium and Calcium, MP Biomedicals
Supplier: MP Biomedicals
Used to minimise cell attachment or aggregation.
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Dulbecco's phosphate buffered saline (DPBS), cell culture, MP Biomedicals
Supplier: MP Biomedicals
Phosphate buffered salts, Dulbecco's formula (DPBS) is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. DPBS is used to to irrigate, wash, and dilute mammalian cells.
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Hank's balanced salts, MP Biomedicals
Supplier: MP Biomedicals
Hanks' balanced salts solution is used to maintain the pH and osmotic balance in the medium and to provide the cells with water and essential inorganic ions.
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Hank's balanced salt solution, modified, MP Biomedicals
Supplier: MP Biomedicals
Cell culture buffer, Hank's balanced salt solution, modified (1X)
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Saline solutions
Supplier: BALIS
Cell culture buffer, Physiological saline solution, 9 ml, no standards
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Phosphate Buffered Saline
Supplier: THERMO FISHER DIAGNOSTICS
Cell culture buffer, Phosphate buffered saline tablets