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Tamponi per colture cellulari

Tissue culture buffers provide an energy source, maintain proper pH levels, and keep ideal osmotic pressure present. Used predominately in biomolecular research, the dry and liquid substances have a balanced inorganic salt composition. Lower concentration options permit equilibration with air in a closed system, while higher salt concentration options keep gaseous phases in equilibrium. Cell growth is optimal when plated in a nutritional medium that has been buffered.

Phosphate-buffered saline (PBS, 10X), pH 7,6

Supplier: Thermo Fisher Scientific

Cell culture buffer, PBS, 10 X

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TE buffer, pH 8,1, RNAse free

Supplier: Thermo Fisher Scientific

Trizma is used in the formulation of buffer solutions in the pH range between 7,5 and 8,5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification.

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Krebs-Ringer solution, bicarbonate-buffered

Supplier: Thermo Fisher Scientific

Contains: 120 mM sodium chloride, 5 mM potassium chloride, 2 mM calcium chloride, 1 mM magnesium chloride, 25 mM sodium bicarbonate, and 5,5 mM D-glucose.

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Sodium bicarbonate, Corning®

Sodium bicarbonate, Corning®

Supplier: Corning

Sodium bicarbonate is used as part of a buffering system commonly used to maintain physiological pH 7,2 to 7,4 in a culture environment

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HEPES Buffer, ES Qualified, HyClone™ AdvanceSTEM™

HEPES Buffer, ES Qualified, HyClone™ AdvanceSTEM™

Supplier: HyClone products (Cytiva)

HyClone AdvanceSTEM ES Qualified HEPES provides maintenance of physiological pH in cell culture despite changes in carbon dioxide.

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HEPES-buffered saline, pH 7,0 (2X for transfection)

Supplier: Thermo Fisher Scientific

Cell culture buffer, HEPES-buffered Saline

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Phosphate-buffered saline (DPBS, 10X), Dulbecco's formula

Supplier: Thermo Fisher Scientific

Cell culture buffer, Phosphate-buffered saline (DPBS, 10X), Dulbecco's formula

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10% SDS (Sodium Dodecyl Sulfate), Corning®

10% SDS (Sodium Dodecyl Sulfate), Corning®

Supplier: Corning

Sodium Dodecyl Sulfate (SDS) is an anionic detergent with applications in SDS-PAGE protein analysis and nucleic acid purification. It is a wetting agent that is effective in both acid and alkaline solutions, and is most often used as a protein and lipid solubilization reagent. SDS is also a powerful protein denaturant. This 10% solution is prepared in 18 megaohm water and is 0.2µm filtered.

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Carbonate-buffered saline (5X), pH 9,5

Supplier: Thermo Fisher Scientific

Cell culture buffer, Carbonate-buffered Salin, 5X

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Phosphate buffered saline, PBS-TWEEN® tablets, Millipore®

Supplier: Merck Millipore (Oncogene)

Developed for use in immunoassay applications.

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Sodium acetate, Corning® 3M™

Sodium acetate, Corning® 3M™

Supplier: Corning

Sodium Acetate is a common molecular biology reagent. All production lots are tested to ensure that they are free of RNAse. It is often used in buffer systems to maintain the pH of a given solution.

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Buffer, HEPES solution (1 mol/L), Corning®

Buffer, HEPES solution (1 mol/L), Corning®

Supplier: Corning

HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulphonic acid) provides an alternative to the use of bicarbonate for buffering because it does not require elevated levels of CO2

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Hanks' Balanced Salts (Modified), 1X, without Magnesium and Calcium, MP Biomedicals

Hanks' Balanced Salts (Modified), 1X, without Magnesium and Calcium, MP Biomedicals

Supplier: MP Biomedicals

Used to minimise cell attachment or aggregation.

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Dulbecco's phosphate buffered saline (DPBS), cell culture, MP Biomedicals

Dulbecco's phosphate buffered saline (DPBS), cell culture, MP Biomedicals

Supplier: MP Biomedicals

Phosphate buffered salts, Dulbecco's formula (DPBS) is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. DPBS is used to to irrigate, wash, and dilute mammalian cells.

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Hank's balanced salts, MP Biomedicals

Supplier: MP Biomedicals

Hanks' balanced salts solution is used to maintain the pH and osmotic balance in the medium and to provide the cells with water and essential inorganic ions.

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Hank's balanced salt solution, modified, MP Biomedicals

Supplier: MP Biomedicals

Cell culture buffer, Hank's balanced salt solution, modified (1X)

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Saline solutions

Supplier: BALIS

Cell culture buffer, Physiological saline solution, 9 ml, no standards

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Phosphate Buffered Saline

Supplier: THERMO FISHER DIAGNOSTICS

Cell culture buffer, Phosphate buffered saline tablets

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