Tamponi per colture cellulari

I tamponi per colture cellulari forniscono una fonte di energia, mantengono livelli adeguati di pH e conservano la pressione osmotica ideale. Utilizzati principalmente nella ricerca biomolecolare, le sostanze secche e liquide presentano una composizione equilibrata di sali inorganici. Le opzioni a bassa concentrazione consentono l’equilibrio con l’aria in un sistema chiuso, mentre quelle ad alta concentrazione salina mantengono le fasi gassose in equilibrio. La crescita cellulare è ottimale quando viene effettuata in un mezzo nutritivo tamponato.

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HEPES-buffered saline, pH 7,0 (2X for transfection)

Supplier: Thermo Fisher Scientific

Cell culture buffer, HEPES-buffered Saline

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Phosphate-buffered saline (PBS, 10X), pH 7,6

Supplier: Thermo Fisher Scientific

Cell culture buffer, PBS, 10 X

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TE buffer, pH 8,1, RNAse free

Supplier: Thermo Fisher Scientific

Trizma is used in the formulation of buffer solutions in the pH range between 7,5 and 8,5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification.

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Carbonate-buffered saline (5X), pH 9,5

Supplier: Thermo Fisher Scientific

Cell culture buffer, Carbonate-buffered Salin, 5X

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Phosphate-buffered saline (DPBS, 10X), Dulbecco's formula

Supplier: Thermo Fisher Scientific

Cell culture buffer, Phosphate-buffered saline (DPBS, 10X), Dulbecco's formula

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Sodium bicarbonate, Corning®

Supplier: Corning

Sodium bicarbonate is used as part of a buffering system commonly used to maintain physiological pH 7,2 to 7,4 in a culture environment

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10X Tris buffered saline, liquid, Corning®

Supplier: Corning

Tris Buffered Saline (TBS) is an isotonic, non-toxic buffer used in some biomedical techniques to maintain pH within a relatively narrow range.

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Sodium acetate, Corning® 3M™

Supplier: Corning

Sodium Acetate is a common molecular biology reagent. All production lots are tested to ensure that they are free of RNAse. It is often used in buffer systems to maintain the pH of a given solution.

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G-Biosciences Phosphate Buffer (200 mM Each)

Supplier: G-Biosciences

Water based salt solution containing sodium phosphate monobasic and dibasic at a fixed pH.

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Krebs-Ringer solution, bicarbonate-buffered

Supplier: Thermo Fisher Scientific

Contains: 120 mM sodium chloride, 5 mM potassium chloride, 2 mM calcium chloride, 1 mM magnesium chloride, 25 mM sodium bicarbonate, and 5,5 mM D-glucose.

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Krebs-Ringer solution, HEPES-buffered

Supplier: Thermo Fisher Scientific

Contains 120 mM sodium chloride, 5 mM potassium chloride, 2 mM calcium chloride, 1 mM magnesium chloride, 25 mM sodium bicarbonate, 5,5 mM HEPES, and 1 mM D-glucose

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Phosphate-buffered saline (DPBS, 1X), Dulbecco's formula, without calcium, without magnesium

Supplier: Thermo Fisher Scientific

Cell culture buffer, DPBS, 1X

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Hank's balanced salts, MP Biomedicals

Supplier: MP Biomedicals

Hanks' balanced salts solution is used to maintain the pH and osmotic balance in the medium and to provide the cells with water and essential inorganic ions.

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Hank's balanced salt solution, modified, MP Biomedicals

Supplier: MP Biomedicals

Cell culture buffer, Hank's balanced salt solution, modified (1X)

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Hanks' Balanced Salts (Modified), 1X, without Magnesium and Calcium, MP Biomedicals

Supplier: MP Biomedicals

Used to minimise cell attachment or aggregation.

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Dulbecco's phosphate buffered saline (DPBS), cell culture, MP Biomedicals

Supplier: MP Biomedicals

Phosphate buffered salts, Dulbecco's formula (DPBS) is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. DPBS is used to to irrigate, wash, and dilute mammalian cells.